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Unravelling physiological signatures of tomato bacterial wilt and xylem metabolites exploited by Ralstonia solanacearum

hal.structure.identifierLaboratoire des Interactions Plantes Microbes Environnement [LIPME]
dc.contributor.authorGERLIN, Léo
hal.structure.identifierLaboratoire des Interactions Plantes Microbes Environnement [LIPME]
dc.contributor.authorESCOURROU, Antoine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCASSAN, Cédric
hal.structure.identifierLaboratoire des Interactions Plantes Microbes Environnement [LIPME]
dc.contributor.authorMACIA, Felicià
hal.structure.identifierLaboratoire des Interactions Plantes Microbes Environnement [LIPME]
dc.contributor.authorPEETERS, Nemo
hal.structure.identifierLaboratoire des Interactions Plantes Microbes Environnement [LIPME]
dc.contributor.authorGENIN, Stéphane
hal.structure.identifierLaboratoire des Interactions Plantes Microbes Environnement [LIPME]
dc.contributor.authorBAROUKH, Caroline
dc.date.issued2021-04-26
dc.identifier.issn1462-2912
dc.description.abstractEnThe plant pathogen Ralstonia solanacearum uses plant resources to intensely proliferate in xylem vessels and provoke plant wilting. We combined automatic phenotyping and tissue/xylem quantitative metabolomics of infected tomato plants to decipher the dynamics of bacterial wilt. Daily acquisition of physiological parameters such as transpiration and growth were performed. Measurements allowed us to identify a tipping point in bacterial wilt dynamics. At this tipping point, the reached bacterial density brutally disrupts plant physiology and rapidly induces its death. We compared the metabolic and physiological signatures of the infection to drought stress, and found that similar changes occur. Quantitative dynamics of xylem content enabled us to identify glutamine (and asparagine) as primary resources R. solanacearum consumed during its colonization phase. An abundant production of putrescine was also observed during the infection process and was strongly correlated with in planta bacterial growth. Dynamic profiling of xylem metabolites confirmed that glutamine is the favored substrate of R. solanacearum. On the other hand, a triple mutant strain unable to metabolize glucose, sucrose and fructose appears to be only weakly reduced for in planta growth and pathogenicity.
dc.description.sponsorshipTowards a Unified theory of biotic Interactions: the roLe of environmental - ANR-10-LABX-0041
dc.description.sponsorshipUniversité Fédérale de Toulouse - ANR-11-IDEX-0002
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
dc.language.isoen
dc.publisherSociety for Applied Microbiology and Wiley-Blackwell
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enmetabolism
dc.subject.enplant pathogen
dc.subject.entranspiration
dc.subject.engrowth
dc.subject.englutamine
dc.subject.enputrescine
dc.subject.enNMR
dc.subject.enmetabolomics
dc.subject.enautomated phenotyping
dc.title.enUnravelling physiological signatures of tomato bacterial wilt and xylem metabolites exploited by Ralstonia solanacearum
dc.typeArticle de revue
dc.identifier.doi10.1111/1462-2920.15535
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Botanique
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologie/Physiologie [q-bio.TO]
bordeaux.journalEnvironmental Microbiology
bordeaux.page5962-5978
bordeaux.volume23
bordeaux.issue10
bordeaux.peerReviewedoui
hal.identifierhal-03211148
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03211148v1
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