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High Resolution Quantitative Trait Locus Mapping and Whole Genome Sequencing Enable the Design of an Anthocyanidin Reductase-Specific Homoeo-Allelic Marker for Fruit Colour Improvement in Octoploid Strawberry (Fragaria × ananassa)

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLABADIE, Marc
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorVALLIN, Guillaume
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPOTIER, Aline
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierInvenio
dc.contributor.authorPETIT, Aurélie
hal.structure.identifierTechnische Universität Munchen - Technical University Munich - Université Technique de Munich [TUM]
dc.contributor.authorRING, Ludwig
hal.structure.identifierTechnische Universität Munchen - Technical University Munich - Université Technique de Munich [TUM]
dc.contributor.authorHOFFMANN, Thomas
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGASTON, Amélia
hal.structure.identifierUniversidad de Córdoba = University of Córdoba [Córdoba]
dc.contributor.authorMUNOZ-BLANCO, Juan
hal.structure.identifierUniversidad de Córdoba = University of Córdoba [Córdoba]
dc.contributor.authorCABALLERO, José
hal.structure.identifierTechnische Universität Munchen - Technical University Munich - Université Technique de Munich [TUM]
dc.contributor.authorSCHWAB, Wilfried
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDENOYES, Béatrice
dc.date.issued2022-03-18
dc.identifier.issn1664-462X
dc.description.abstractEnFruit colour is central to the sensorial and nutritional quality of strawberry fruit and is therefore a major target in breeding programmes of the octoploid cultivated strawberry (Fragaria × ananassa). The red colour of the fruit is caused by the accumulation of anthocyanins, which are water-soluble flavonoids. To facilitate molecular breeding, here we have mapped with high resolution fruit colour quantitative trait loci (QTLs) (COLOUR, scored visually as in selection programmes) and associated flavonoid metabolic QTLs (5 anthocyanins compounds together with 8 flavonols and flavan-3-ols) to specific subgenomes of cultivated strawberry. Two main colour-related QTLs were located on the LG3A linkage group (Fragaria vesca subgenome). Genetic mapping, transcriptome analysis and whole genome sequencing enabled the detection of a homoeo-allelic variant of ANTHOCYANIDIN REDUCTASE (ANR) underlying the major male M3A COLOUR and pelargonidin-3-glucoside (PgGs) QTLs (up to ∼20% of explained variance). Consistent with previously published functional studies, ANR transcript abundance was inversely related with PgGs content in contrasted progeny individuals. Genetic segregation analyses further indicated that a molecular marker designed using an 18 bp deletion found in the 5 UTR of the candidate ANR homoeoallelic variant is effective in identifying genotypes with intense red fruit colour. Our study provides insights into the genetic and molecular control of colour-related traits in strawberry and further defines a genetic marker for marker-assisted selection of new strawberry varieties with improved colour. The QTLs detected and the underlying candidate genes are different from those described to date, emphasising the importance of screening a wide diversity of genetic resources in strawberry.
dc.language.isoen
dc.publisherFrontiers
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectFruit
dc.subjectplante fruitière
dc.subject.enFragaria × ananassa
dc.subject.encolour
dc.subject.enanthocyanins
dc.subject.enmQTL
dc.subject.enF. vesca subgenome
dc.subject.enhomoeo-allele
dc.subject.enANR
dc.subject.enMYBlike ODORANT
dc.title.enHigh Resolution Quantitative Trait Locus Mapping and Whole Genome Sequencing Enable the Design of an Anthocyanidin Reductase-Specific Homoeo-Allelic Marker for Fruit Colour Improvement in Octoploid Strawberry (Fragaria × ananassa)
dc.typeArticle de revue
dc.identifier.doi10.3389/fpls.2022.869655
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Amélioration des plantes
dc.description.sponsorshipEuropeGoodBerry project
dc.description.sponsorshipEuropeEuropean Union’s ERANET
bordeaux.journalFrontiers in Plant Science
bordeaux.page869655
bordeaux.volume13
bordeaux.peerReviewedoui
hal.identifierhal-03626030
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03626030v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers%20in%20Plant%20Science&rft.date=2022-03-18&rft.volume=13&rft.spage=869655&rft.epage=869655&rft.eissn=1664-462X&rft.issn=1664-462X&rft.au=LABADIE,%20Marc&VALLIN,%20Guillaume&POTIER,%20Aline&PETIT,%20Aur%C3%A9lie&RING,%20Ludwig&rft.genre=article


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