| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | MAZORRA MORALES, Luis Miguel | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | COSME SILVA, Gláucia Michelle | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | SANTANA, Diederson Bortolini | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | PIREDA, Saulo | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | DORIGHETTO COGO, Antônio Jesus | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | HERINGER, Ângelo Schuabb | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | DE OLIVEIRA, Tadeu Dos Reis | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | REIS, Ricardo | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | DOS SANTOS PRADO, Luís Alfredo | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | DE OLIVEIRA, André Vicente | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | SILVEIRA, Vanildo | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | DA CUNHA, Maura | |
| hal.structure.identifier | Instituto de Pesquisas Jardim Botanico do Rio de Janeiro [JBRJ] | |
| dc.contributor.author | BARROS, Cláudia | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | FAÇANHA, Arnoldo | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | BALDET, Pierre | |
| hal.structure.identifier | Universidad Nacional de la Plata [Argentine] [UNLP] | |
| dc.contributor.author | BARTOLI, Carlos | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | DA SILVA, Marcelo Gomes | |
| hal.structure.identifier | Universidade Estadual do Norte Fluminense Darcy Ribeiro [UENF] | |
| dc.contributor.author | OLIVEIRA, Jurandi | |
| dc.date.issued | 2022-08 | |
| dc.identifier.issn | 0981-9428 | |
| dc.description.abstractEn | Mitochondria are the major organelles of energy production; however, active mitochondria can decline their energetic role and show a dysfunctional status. Mitochondrial dysfunction was induced by high nonphysiological level of L-galactone-1,4-lactone (L-GalL), the precursor of ascorbate (AsA), in plant mitochondria. The dysfunction induced by L-GalL was associated with the fault in the mitochondrial electron partition and reactive oxygen species (ROS) over-production. Using mitochondria from RNAi-plant lines harbouring silenced Lgalactone-1,4-lactone dehydrogenase (L-GalLDH) activity, it was demonstrated that such dysfunction is dependent on this enzyme activity. The capacity of alternative respiration was strongly decreased by L-GalL, probably mediated by redox-inactivation of the alternative oxidase (AOX) enzyme. Although, alternative respiration was shown to be the key factor that helps support AsA synthesis in dysfunctional mitochondria. Experiments with respiratory inhibitors showed that ROS formation and mitochondrial dysfunction were more associated with the decline in the activities of COX (cytochrome oxidase) and particularly AOX than with the lower activities of respiratory complexes I and III. The application of high L-GalL concentrations induced proteomic changes that indicated alterations in proteins related to oxidative stress and energetic status. However, supra-optimal L-GalL concentration was not deleterious for plants. Instead, the L-GalLDH activity could be positive. Indeed, it was found that wild type plants performed better growth than L-GalLDH-RNAi plants in response to high nonphysiological L-GalL concentrations. | |
| dc.language.iso | en | |
| dc.subject.en | Alternative oxidase | |
| dc.subject.en | H2O2 | |
| dc.subject.en | L-galactone-1 | |
| dc.subject.en | 4-lactone dehydrogenase | |
| dc.subject.en | 4-lactone | |
| dc.subject.en | Mitochondrial electron transport chain | |
| dc.subject.en | Reactive oxygen species | |
| dc.title.en | Mitochondrial dysfunction associated with ascorbate synthesis in plants | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1016/j.plaphy.2022.05.025 | |
| dc.subject.hal | Sciences du Vivant [q-bio] | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | |
| bordeaux.journal | Plant Physiology and Biochemistry | |
| bordeaux.page | 55-68 | |
| bordeaux.volume | 185 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-03753348 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-03753348v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Physiology%20and%20Biochemistry&rft.date=2022-08&rft.volume=185&rft.spage=55-68&rft.epage=55-68&rft.eissn=0981-9428&rft.issn=0981-9428&rft.au=MAZORRA%20MORALES,%20Luis%20Miguel&COSME%20SILVA,%20Gl%C3%A1ucia%20Michelle&SANTANA,%20Diederson%20Bortolini&PIREDA,%20Saulo&DORIGHETTO%20COGO,%20Ant%C3%B4ni&rft.genre=article | |
