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Direct imaging of lateral movements of AMPA receptors inside synapses.

hal.structure.identifierCentre de physique moléculaire optique et hertzienne [CPMOH]
dc.contributor.authorTARDIN, Catherine
hal.structure.identifierCentre de physique moléculaire optique et hertzienne [CPMOH]
dc.contributor.authorCOGNET, Laurent
hal.structure.identifierPhysiologie cellulaire de la synapse [PCS]
dc.contributor.authorBATS, Cécile
hal.structure.identifierCentre de physique moléculaire optique et hertzienne [CPMOH]
dc.contributor.authorLOUNIS, Brahim
hal.structure.identifierPhysiologie cellulaire de la synapse [PCS]
dc.contributor.authorCHOQUET, Daniel
dc.date.issued2003-09-15
dc.identifier.issn0261-4189
dc.description.abstractEnTrafficking of AMPA receptors in and out of synapses is crucial for synaptic plasticity. Previous studies have focused on the role of endo/exocytosis processes or that of lateral diffusion of extra-synaptic receptors. We have now directly imaged AMPAR movements inside and outside synapses of live neurons using single-molecule fluorescence microscopy. Inside individual synapses, we found immobile and mobile receptors, which display restricted diffusion. Extra-synaptic receptors display free diffusion. Receptors could also exchange between these membrane compartments through lateral diffusion. Glutamate application increased both receptor mobility inside synapses and the fraction of mobile receptors present in a juxtasynaptic region. Block of inhibitory transmission to favor excitatory synaptic activity induced a transient increase in the fraction of mobile receptors and a decrease in the proportion of juxtasynaptic receptors. Altogether, our data show that rapid exchange of receptors between a synaptic and extra-synaptic localization occurs through regulation of receptor diffusion inside synapses.
dc.language.isoen
dc.publisherEMBO Press
dc.subject.meshAnimals
dc.subject.meshBicuculline
dc.subject.meshNeuronal Plasticity
dc.subject.meshNeurons
dc.subject.meshRats
dc.subject.meshSynapses
dc.subject.meshTetrodotoxin
dc.subject.meshCells, Cultured
dc.subject.meshDiffusion
dc.subject.meshEmbryo
dc.subject.meshGlucose Transporter Type 2
dc.subject.meshGlutamic Acid
dc.subject.meshHippocampus
dc.subject.meshMicroscopy, Fluorescence
dc.subject.meshMonosaccharide Transport Proteins
dc.title.enDirect imaging of lateral movements of AMPA receptors inside synapses.
dc.typeArticle de revue
dc.identifier.doi10.1093/emboj/cdg463
dc.subject.halPhysique [physics]/Physique [physics]/Optique [physics.optics]
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]/Neurobiologie
dc.identifier.arxiv0704.3858
bordeaux.journalEMBO Journal
bordeaux.page4656-65
bordeaux.volume22
bordeaux.issue18
bordeaux.peerReviewedoui
hal.identifierhal-00143981
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00143981v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=EMBO%20Journal&rft.date=2003-09-15&rft.volume=22&rft.issue=18&rft.spage=4656-65&rft.epage=4656-65&rft.eissn=0261-4189&rft.issn=0261-4189&rft.au=TARDIN,%20Catherine&COGNET,%20Laurent&BATS,%20C%C3%A9cile&LOUNIS,%20Brahim&CHOQUET,%20Daniel&rft.genre=article


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