Afficher la notice abrégée

Silica Nanoparticles Assisted Electrochemical Biosensor for the Detection and Degradation of Escherichia Coli Bacteria

hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorMATHELIÉ-GUINLET, Marion
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorGAMMOUDI, Ibtissem
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEVEN, Laure
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorMOROTÉ, Fabien
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorDELVILLE, Marie-Hélène
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorGRAUBY-HEYWANG, Christine
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorCOHEN-BOUHACINA, Touria
dc.contributor.editorIstván Bársony
dc.contributor.editorZsolt Zolnai
dc.contributor.editorGábor Battistig (Ed.)
dc.date.issued2016
dc.identifier.issn1877-7058
dc.description.abstractEnAn electrochemical biosensor, based on an amplification method using nanoparticles, is being developed for bacteria detection. Firstly, silica nanoparticles with different sizes were synthesized and studied in interaction with Escherichia coli cells for their potential toxicity, by atomic force microscopy and viability tests. A critical diameter in the range of 50-70 nm was found under which nanoparticles triggered drastic membrane damage even leading to cell lysis. Secondly, harmless nanoparticles were used in combination with polyelectrolytes as a transducer. A two steps spin coating protocol enabled (i) to immobilize a polyelectrolytes multilayer and (ii) to adsorb a 100 nm – NPs solution at 1g.L-1. Lastly, we plan to conjugate these nanoparticles with antibodies specific to Escherichia coli for their selective and sensitive detection through an electrochemical sensor.
dc.language.isoen
dc.publisherElsevier
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/
dc.subject.enAFM
dc.subject.enbiosensor
dc.subject.ennanoparticles
dc.subject.entoxicity
dc.subject.enbacteria
dc.title.enSilica Nanoparticles Assisted Electrochemical Biosensor for the Detection and Degradation of Escherichia Coli Bacteria
dc.typeArticle de revue
dc.identifier.doi10.1016/j.proeng.2016.11.337
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.journalProcedia Engineering
bordeaux.page1048 - 1051
bordeaux.volume168
bordeaux.peerReviewedoui
hal.identifierhal-01477777
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01477777v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Procedia%20Engineering&rft.date=2016&rft.volume=168&rft.spage=1048%20-%201051&rft.epage=1048%20-%201051&rft.eissn=1877-7058&rft.issn=1877-7058&rft.au=MATHELI%C3%89-GUINLET,%20Marion&GAMMOUDI,%20Ibtissem&BEVEN,%20Laure&MOROT%C3%89,%20Fabien&DELVILLE,%20Marie-H%C3%A9l%C3%A8ne&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée