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Prestressed cells are prone to cytoskeleton failures under localized shear strain: an experimental demonstration on muscle precursor cells

hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
hal.structure.identifierLaboratoire de biologie et modélisation de la cellule [LBMC UMR 5239]
dc.contributor.authorSTREPPA, Laura
hal.structure.identifierLaboratoire de biologie et modélisation de la cellule [LBMC UMR 5239]
dc.contributor.authorRATTI, Francesca
hal.structure.identifierLaboratoire de biologie et modélisation de la cellule [LBMC UMR 5239]
dc.contributor.authorGOILLOT, Evelyne
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorDEVIN, Anne
hal.structure.identifierLaboratoire de biologie et modélisation de la cellule [LBMC UMR 5239]
dc.contributor.authorSCHAEFFER, Laurent
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
dc.contributor.authorARNEODO, Alain
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
dc.contributor.authorARGOUL, Françoise
dc.date.created2017-08-02
dc.date.issued2018-12
dc.identifier.issn2045-2322
dc.description.abstractEnWe report on a wavelet based space-scale decomposition method for analyzing the response of living muscle precursor cells (C2C12 myoblasts and myotubes) upon sharp indentation with an AFM cantilever and quantifying their aptitude to sustain such a local shear strain. Beyond global mechanical parameters which are currently used as markers of cell contractility, we emphasize the necessity of characterizing more closely the local fluctuations of the shear relaxation modulus as they carry important clues about the mechanisms of cytoskeleton strain release. Rupture events encountered during fixed velocity shear strain are interpreted as local disruptions of the actin cytoskeleton structures, the strongest (brittle) ones being produced by the tighter and stiffer stress fibers or actin agglomerates. These local strain induced failures are important characteristics of the resilience of these cells, and their aptitude to maintain their shape via a quick recovery from local strains. This study focuses on the perinuclear region because it can be considered as a master mechanical organizing center of these muscle precursor cells. Using this wavelet-based method, we combine the global and local approaches for a comparative analysis of the mechanical parameters of normal myoblasts, myotubes and myoblasts treated with actomyosin cytoskeleton disruptive agents (ATP depletion, blebbistatin).
dc.description.sponsorshipPhysique de la morphogenèse des plantes: propriétés dynamiques et mécaniques de la paroi des cellules du méristème
dc.language.isoen
dc.publisherNature Publishing Group
dc.title.enPrestressed cells are prone to cytoskeleton failures under localized shear strain: an experimental demonstration on muscle precursor cells
dc.typeArticle de revue
dc.identifier.doi10.1038/s41598-018-26797-4
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
dc.subject.halPhysique [physics]/Physique [physics]/Physique Médicale [physics.med-ph]
dc.subject.halPhysique [physics]/Mécanique [physics]/Biomécanique [physics.med-ph]
dc.subject.halSciences du Vivant [q-bio]/Biologie cellulaire/Interactions cellulaires [q-bio.CB]
dc.subject.halSciences du Vivant [q-bio]/Bio-Informatique, Biologie Systémique [q-bio.QM]
bordeaux.journalScientific Reports
bordeaux.page8602(1-16)
bordeaux.volume8
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-01807908
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01807908v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2018-12&rft.volume=8&rft.issue=1&rft.spage=8602(1-16)&rft.epage=8602(1-16)&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=STREPPA,%20Laura&RATTI,%20Francesca&GOILLOT,%20Evelyne&DEVIN,%20Anne&SCHAEFFER,%20Laurent&rft.genre=article


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