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hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMATHELIÉ-GUINLET, Marion
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorCOHEN-BOUHACINA, Touria
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorGAMMOUDI, Ibtissem
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorMARTIN, Axel
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEVEN, Laure
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorDELVILLE, Marie-Hélène
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorGRAUBY-HEYWANG, Christine
dc.date.issued2019
dc.identifier.issn0925-4005
dc.description.abstractEnTo face the emerging issues raised by bacterial infections in biomedical, food and environmental contexts, the combination of nanotechnologies and biosensors characteristics is increasingly seen as a promising tool to develop rapid, highly sensitive and specific devices for reliable bacterial detection and quantification. We present here the design of an electrochemical immuno-biosensor, integrating non cytotoxic silica nanoparticles (NPs), in a two-step spin coating process. Unlike biosensors that integrate a unique antibody-based layer without NPs, this new tool allows, through cyclic voltammetry measurements, the continuous and non-saturated detection of E. coli bacteria in only 5 min. and can reach up to 103 CFU/mL in 30 min. It offers great promises for industrial purposes as electrode functionalization is rather simple and its architecture can be tuned on demand to target a variety of micro-organisms, and the highlighted indicators (variations of current intensity, intensity ratios and/or difference of potentials) can easily be implemented in a screen displayed packaging.
dc.language.isoen
dc.publisherElsevier
dc.subject.enNanoparticles
dc.subject.enImmunosensor
dc.subject.enCyclic voltammetry
dc.subject.enE. coli
dc.title.enSilica nanoparticles-assisted electrochemical biosensor for the rapid, sensitive and specific detection of Escherichia coli
dc.typeArticle de revue
dc.identifier.doi10.1016/j.snb.2019.03.144
dc.subject.halChimie/Autre
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
bordeaux.journalSensors and Actuators B: Chemical
bordeaux.page314-320
bordeaux.volume292
bordeaux.peerReviewedoui
hal.identifierhal-02123016
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02123016v1
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