In Vivo Therapeutic Silencing of Hypoxia-Inducible Factor 1 Alpha (HIF-1\alpha) Using Single-Walled Carbon Nanotubes Noncovalently Coated with siRNA
| hal.structure.identifier | Department of Experimental Therapeutics | |
| dc.contributor.author | BARTHOLOMEUSZ, Geoffrey | |
| hal.structure.identifier | Department of Experimental Therapeutics | |
| hal.structure.identifier | Department of Chemistry | |
| dc.contributor.author | CHERUKURI, Paul | |
| hal.structure.identifier | Department of Experimental Therapeutics | |
| dc.contributor.author | KINGSTON, John | |
| hal.structure.identifier | Department of Chemistry | |
| hal.structure.identifier | Centre de physique moléculaire optique et hertzienne [CPMOH] | |
| dc.contributor.author | COGNET, Laurent | |
| hal.structure.identifier | Department of Experimental Therapeutics | |
| dc.contributor.author | LEMOS, Robert Jr. | |
| hal.structure.identifier | Department of Chemistry | |
| dc.contributor.author | LEEUW, Tonya | |
| hal.structure.identifier | Department of Experimental Therapeutics | |
| dc.contributor.author | GUMBINER-RUSSO, Laura | |
| hal.structure.identifier | Department of Chemistry | |
| dc.contributor.author | WEISMAN, R. Bruce | |
| hal.structure.identifier | Department of Experimental Therapeutics | |
| dc.contributor.author | POWIS, Garth | |
| dc.date.created | 2008-12-11 | |
| dc.date.issued | 2009-04-17 | |
| dc.identifier.issn | 1998-0124 | |
| dc.description.abstractEn | A new approach is described for delivering small interfering RNA (siRNA) into cancer cells by noncovalently complexing unmodifi ed siRNA with pristine single-walled carbon nanotubes (SWCNTs). The complexes were prepared by simple sonication of pristine SWCNTs in a solution of siRNA, which then served both as the cargo and as the suspending agent for the SWCNTs. When complexes containing siRNA targeted to hypoxiainducible factor 1 alpha (HIF-1\alpha) were added to cells growing in serum containing culture media, there was strong specific inhibition of cellular HIF-1\alpha activity. The ability to obtain a biological response to SWCNT/ siRNA complexes was seen in a wide variety of cancer cell types. Moreover, intratumoral administration of SWCNT- HIF-1\alpha siRNA complexes in mice bearing MiaPaCa-2 /HRE tumors signifi cantly inhibited the activity of tumor HIF-1 . As elevated levels of HIF-1\alpha are found in many human cancers and are associated with resistance to therapy and decreased patient survival, these results imply that SWCNT/siRNA complexes may have value as therapeutic agents. | |
| dc.language.iso | en | |
| dc.publisher | Springer | |
| dc.subject.en | siRNA | |
| dc.subject.en | single-walled carbon nanotubes | |
| dc.subject.en | anti-cancer therapy | |
| dc.subject.en | in vivo delivery agent | |
| dc.title.en | In Vivo Therapeutic Silencing of Hypoxia-Inducible Factor 1 Alpha (HIF-1\alpha) Using Single-Walled Carbon Nanotubes Noncovalently Coated with siRNA | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1007/s12274-009-9026-7 | |
| bordeaux.journal | Nano Research | |
| bordeaux.page | 279-291 | |
| bordeaux.volume | 2 | |
| bordeaux.issue | 4 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-00505889 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-00505889v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nano%20Research&rft.date=2009-04-17&rft.volume=2&rft.issue=4&rft.spage=279-291&rft.epage=279-291&rft.eissn=1998-0124&rft.issn=1998-0124&rft.au=BARTHOLOMEUSZ,%20Geoffrey&CHERUKURI,%20Paul&KINGSTON,%20John&COGNET,%20Laurent&LEMOS,%20Robert%20Jr.&rft.genre=article |
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