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Target sequence capture in the Brazil nut family (Lecythidaceae): Marker selection and in silico capture from genome skimming data

hal.structure.identifierDepartment of Ecology and Evolutionary Biology
dc.contributor.authorVARGAS, Oscar M.
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorHEUERTZ, Myriam
hal.structure.identifierDepartment of Ecology and Evolutionary Biology
dc.contributor.authorSMITH, Stephen A.
hal.structure.identifierDepartment of Ecology and Evolutionary Biology
hal.structure.identifierSmithsonian Tropical Research Institute
dc.contributor.authorDICK, Christopher W.
dc.date.issued2019
dc.identifier.issn1055-7903
dc.description.abstractEnReconstructing species trees from multi-loci datasets is becoming a standard practice in phylogenetics. Nevertheless, access to high-throughput sequencing may be costly, especially with studies of many samples. The potential high cost makes a priori assessments desirable in order to make informed decisions about sequencing. We generated twelve transcriptomes for ten species of the Brazil nut family (Lecythidaceae), identified a set of putatively orthologous nuclear loci and evaluated, in silico, their phylogenetic utility using genome skimming data of 24 species. We designed the markers using MarkerMiner, and developed a script, GoldFinder, to efficiently sub-select the best makers for sequencing. We captured, in silico, all designed 354 nuclear loci and performed a maximum likelihood phylogenetic analysis on the concatenated sequence matrix. We also calculated individual gene trees with maximum likelihood and used them for a coalescent-based species tree inference. Both analyses resulted in almost identical topologies. However, our nuclear-loci phylogenies were strongly incongruent with a published plastome phylogeny, suggesting that plastome data alone is not sufficient for species tree estimation. Our results suggest that using hundreds of nuclear markers (i.e. 354) will significantly improve the Lecythidaceae species tree. The framework described here will be useful, generally, for developing markers for species tree inference.
dc.description.sponsorshipCEnter of the study of Biodiversity in Amazonia - ANR-10-LABX-0025
dc.language.isoen
dc.publisherElsevier
dc.subjecttransciptomes
dc.subject.enLecythidaceae
dc.subject.enmarkers
dc.subject.enMarkerMiner
dc.subject.enspecies tree
dc.subject.entarget sequencing
dc.title.enTarget sequence capture in the Brazil nut family (Lecythidaceae): Marker selection and in silico capture from genome skimming data
dc.typeArticle de revue
dc.identifier.doi10.1016/j.ympev.2019.02.020
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalMolecular Phylogenetics and Evolution
bordeaux.page98-104
bordeaux.volume135
bordeaux.peerReviewedoui
hal.identifierhal-02619793
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02619793v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20Phylogenetics%20and%20Evolution&rft.date=2019&rft.volume=135&rft.spage=98-104&rft.epage=98-104&rft.eissn=1055-7903&rft.issn=1055-7903&rft.au=VARGAS,%20Oscar%20M.&HEUERTZ,%20Myriam&SMITH,%20Stephen%20A.&DICK,%20Christopher%20W.&rft.genre=article


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