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Secretome of the free-living mycelium from the ectomycorrhizal basidiomycete Laccaria bicolor

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorVINCENT, Delphine
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorKOHLER, Annegret
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorCLAVEROL, Stéphane
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorSOLIER, Emilie
hal.structure.identifierInstitut National de la Recherche Agronomique [INRA]
dc.contributor.authorJOETS, Johann
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorGIBON, Julien
hal.structure.identifierPhysiologie des plantes et des champignons lors de l'infection
dc.contributor.authorLEBRUN, Marc-Henri
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPLOMION, Christophe
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorMARTIN, Francis
dc.date.issued2012
dc.identifier.issn1535-3893
dc.description.abstractEnThe ectomycorrhizal basidiomycete Laccaria bicolor has a dual lifestyle with a transitory soil saprotrophic phase and a longer mutualistic interaction with tree roots. Recent evidence suggests that secreted proteins play key roles in host plant colonisation and symbiosis development. However, a limited number of secreted proteins have been characterized, and the full spectrum of effectors involved in the mycobiont invasion and survival remains unknown. We analyzed the extracellular proteins secreted in growth medium by free-living mycelium of L. bicolor as a proxy for its saprotrophic phase. The proteomic analyses (two-dimensional electrophoresis and shotgun proteomics) were substantiated by whole-genome expression transcript profiling on ectomycorrhizal roots. Among the 224 proteins identified were carbohydrate-acting enzymes likely involved in the cell wall remodelling linked to hyphal growth as well as secreted proteases possibly digesting soil organic compounds and/or fending off competitors, pathogens, and predators. Evidence of gene expression was found in ectomycorrhizal roots for 210 of them. These findings provide the first global view of the secretome of a mutualistic symbiont and shed some light on the mechanisms controlling cell wall remodelling during the hyphal growth. They also revealed many novel putative secreted proteins of unknown function, including one mycorrhiza-induced small secreted protein.
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.subject2-DE
dc.subjectsymbiosis
dc.subjectlaccaire
dc.subject.enectomycorrhizal fungus
dc.subject.enLaccaria bicolor
dc.subject.enmycelium
dc.subject.ensecreted proteins
dc.subject.ensecretomic
dc.subject.enshotgun proteomics
dc.subject.enchampignon forestier
dc.title.enSecretome of the free-living mycelium from the ectomycorrhizal basidiomycete Laccaria bicolor
dc.typeArticle de revue
dc.identifier.doi10.1021/pr200895f
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
bordeaux.journalJournal of Proteome Research
bordeaux.page157-171
bordeaux.volume11
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-02646860
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02646860v1
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