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Cryopreservation of Prunus cerasus through vitrification and replacement of cold hardening with preculture on medium enriched with sucrose and/or glycerol

hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
hal.structure.identifierInstitut de Recherche pour le Développement [IRD]
dc.contributor.authorBARRACO, Giuseppe
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
hal.structure.identifierInstitut de Recherche pour le Développement [IRD]
dc.contributor.authorCHATELET, Philippe
hal.structure.identifierUnité de recherches Espèces Fruitières et Vigne [UREFV]
dc.contributor.authorBALSEMIN, Emilie
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorDECOURCELLE, Thibaut
hal.structure.identifierInstitut de Recherche pour le Développement [IRD]
dc.contributor.authorSYLVESTRE, Isabelle
hal.structure.identifierInstitut de Recherche pour le Développement [IRD]
dc.contributor.authorENGELMANN, Florent
dc.date.issued2012
dc.identifier.issn0304-4238
dc.description.abstractEnCryopreservation is the only alternative, safe and cost-effective method for long-term storage of plant genetic resources, particularly for stone fruits (Prunus spp.). In this study, an efficient cryopreservation protocol was developed for sour cherry (Prunus cerasus L.). In vitro shoot tips of two varieties (Montmorency and Schattenmorelle) were successfully cryopreserved using the vitrification technique. Our study showed the possibility of replacing the 3-week cold hardening treatment of mother-plants with preculture of apices on medium enriched with sucrose and/or glycerol. The highest recovery percentages after liquid nitrogen exposure were obtained after a cold hardening treatment followed by a 3-day preculrure on 0.8 M sucrose medium or by replacing cold hardening with a 7-day preculture on the following media: 0.4 M glycerol or sucrose, 0.4 M sucrose + 0.4 M glycerol or 0.8 M glycerol. Under these conditions, recovery after cryopreservation ranged between 41 and 63%, These results complement the range of Prunus species successfully cryopreserved using in vitro explants. Our protocol, which is simplified in comparison with the original one, since cold hardening of mother-plants in a cold chamber is replaced by pretreatment of apices on medium with high sugar concentration, may facilitate the application of vitrification for cryopreservation of additional Prunus species
dc.language.isoen
dc.publisherElsevier
dc.subject.encold hardening
dc.subject.encryopreservation
dc.subject.englycerol
dc.subject.enpreculture
dc.subject.ensucrose
dc.subject.envitrification
dc.title.enCryopreservation of Prunus cerasus through vitrification and replacement of cold hardening with preculture on medium enriched with sucrose and/or glycerol
dc.typeArticle de revue
dc.identifier.doi10.1016/j.scienta.2012.09.034
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Ingénierie des aliments
dc.subject.halSciences de l'ingénieur [physics]/Génie des procédés
bordeaux.journalScientia Horticulturae
bordeaux.page104-108
bordeaux.volume148
bordeaux.peerReviewedoui
hal.identifierhal-02647241
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02647241v1
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