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Comparative analysis of secreted protein evolution using expressed sequence tags from four poplar leaf rusts (Melampsora spp.)

hal.structure.identifierNatural Resources Canada [NRCan]
dc.contributor.authorJOLY, David L.
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierNatural Resources Canada [NRCan]
dc.contributor.authorFEAU, Nicolas
hal.structure.identifierNatural Resources Canada [NRCan]
dc.contributor.authorTANGUAY, Philippe
hal.structure.identifierNatural Resources Canada [NRCan]
hal.structure.identifierUniversity of British Columbia [UBC]
dc.contributor.authorHAMELIN, Richard
dc.date.issued2010
dc.identifier.issn1471-2164
dc.description.abstractEnBackground: Obligate biotrophs such as rust fungi are believed to establish long-term relationships by modulating plant defenses through a plethora of effector proteins, whose most recognizable feature is the presence of a signal peptide for secretion. Since the phenotypes of these effectors extend to host cells, their genes are expected to be under accelerated evolution stimulated by host-pathogen coevolutionary arms races. Recently, whole genome sequence data has allowed the prediction of secretomes, facilitating the identification of putative effectors. Results: We generated cDNA libraries from four poplar leaf rust pathogens (Melampsora spp.) and used computational approaches to identify and annotate putative secreted proteins with the aim of uncovering new knowledge about the nature and evolution of the rust secretome. While more than half of the predicted secretome members encoded lineage-specific proteins, similarities with experimentally characterized fungal effectors were also identified. A SAGE analysis indicated a strong stage-specific regulation of transcripts encoding secreted proteins. The average sequence identity of putative secreted proteins to their closest orthologs in the wheat stem rust Puccinia graminis f. sp. tritici was dramatically reduced compared with non-secreted ones. A comparative genomics approach based on homologous gene groups unravelled positive selection in putative members of the secretome. Conclusion: We uncovered robust evidence that different evolutionary constraints are acting on the rust secretome when compared to the rest of the genome. These results are consistent with the view that these genes are more likely to exhibit an effector activity and be involved in coevolutionary arms races with host factors.
dc.language.isoen
dc.publisherBioMed Central
dc.subjectMELAMPSORA SPP.
dc.subjectPEUPLIER
dc.subjectBANQUE DE DONNÉES D'ADN
dc.title.enComparative analysis of secreted protein evolution using expressed sequence tags from four poplar leaf rusts (Melampsora spp.)
dc.typeArticle de revue
dc.identifier.doi10.1186/1471-2164-11-422
dc.subject.halSciences du Vivant [q-bio]/Génétique
bordeaux.journalBMC Genomics
bordeaux.page16 p.
bordeaux.volume11
bordeaux.peerReviewedoui
hal.identifierhal-02660329
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02660329v1
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