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Leaf proteome analysis of eight Populus xeuramericana genotypes : genetic variation in drought response and in water-use efficiency involves photosynthesis-related proteins

hal.structure.identifierGénétique Quantitative et Evolution - Le Moulon (Génétique Végétale) [GQE-Le Moulon]
hal.structure.identifierLaboratoire de Biologie des Ligneux et des Grandes Cultures [LBLGC]
dc.contributor.authorBONHOMME, Ludovic
hal.structure.identifierArbres et Réponses aux Contraintes Hydriques et Environnementales [ARCHE]
hal.structure.identifierLaboratoire de Biologie des Ligneux et des Grandes Cultures [LBLGC]
dc.contributor.authorMONCLUS, Romain
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorVINCENT, Delphine
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorCARPIN, Sabine
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorLOMENECH, Anne-Marie
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPLOMION, Christophe
hal.structure.identifierArbres et Réponses aux Contraintes Hydriques et Environnementales [ARCHE]
dc.contributor.authorBRIGNOLAS, Franck
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorMORABITO, Domenico
dc.date.issued2009
dc.identifier.issn1615-9853
dc.description.abstractEnGenetic variation of leaf proteome in drought response was investigated among eight Populus ×euramericana genotypes contrasting for their leaf carbon isotope discrimination (Δ), an estimate of intrinsic water-use efficiency. Plants were grown in open field on two similar plots. Drought was induced by an 86-day irrigation cessation on one plot, whereas a second plot remained regularly irrigated. Using 2-DE, 863 reproducible spots were detected; about 60% presented at least one significant effect i.e. treatment, genotype and/or genotype by treatment interaction effect. A significant genotype by treatment interaction was detected for 62 reliably identified proteins among which, about 65% consisted in chloroplast-associated proteins either involved in the Calvin cycle or in the electron-transport chains. The other proteins were involved in oxidative stress, amino acid or protein metabolisms. Correlations between protein abundance and Δ variations were found for 45 reliably identified proteins. The abundance of ribulose-1,5-bisphosphate carboxylase/oxygenase activase isoforms scaled negatively with Δ regardless of the treatment, suggesting that a large intrinsic water-use efficiency could be due to higher abundance of ribulose-1,5-bisphosphate carboxylase/oxygenase activase. Under control condition, abundance of enzymes involved in carbon fixation was also negatively correlated with Δ, whereas abundance of enzymes involved in photorespiration or respiration was positively correlated with Δ.
dc.language.isoen
dc.publisherWiley-VCH Verlag
dc.subjectPLANT PROTEOMICS
dc.subjectPOPLAR
dc.subjectPEUPLIER
dc.subject.enCARBON ISOTOPE DISCRIMINATION
dc.subject.enCHLOROPLAST
dc.subject.enOPEN FIELD EXPERIMENT
dc.subject.enRIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE ACTIVASE
dc.title.enLeaf proteome analysis of eight Populus xeuramericana genotypes : genetic variation in drought response and in water-use efficiency involves photosynthesis-related proteins
dc.typeArticle de revue
dc.identifier.doi10.1002/pmic.200900047
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
bordeaux.journalProteomics
bordeaux.page4121-4142
bordeaux.volume9
bordeaux.issue17
bordeaux.peerReviewedoui
hal.identifierhal-02666973
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02666973v1
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