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Towards functional genomics of transcription factor genes associated to growth and wood formation in maritime pine

hal.structure.identifierInstitut Technologique Forêt Cellulose Bois-construction Ameublement [FCBA]
dc.contributor.authorTRONTIN, Jean-François
hal.structure.identifierUniversidad de Málaga [Málaga] = University of Málaga [Málaga]
dc.contributor.authorAVILA, Concepción
hal.structure.identifierInstitut Technologique Forêt Cellulose Bois-construction Ameublement [FCBA]
dc.contributor.authorDEBILLE, Sandrine
hal.structure.identifierUniversidad de Málaga [Málaga] = University of Málaga [Málaga]
dc.contributor.authorDE LA TORRE, Fernando
hal.structure.identifierUniversidad de Málaga [Málaga] = University of Málaga [Málaga]
dc.contributor.authorEL-AZAZ, Jorge
hal.structure.identifierUniversidad de Málaga [Málaga] = University of Málaga [Málaga]
dc.contributor.authorPASCUAL, Belen
hal.structure.identifierInstitut Technologique Forêt Cellulose Bois-construction Ameublement [FCBA]
dc.contributor.authorCANLET, Francis
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorTEYSSIER, Caroline
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorBOIZOT, Nathalie
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorLE METTE, Claire
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorLESAGE DESCAUSES, Marie-Claude
hal.structure.identifierInstitut Technologique Forêt Cellulose Bois-construction Ameublement [FCBA]
dc.contributor.authorDA SILVA PEREZ, Denilson
hal.structure.identifierUniversidad de Málaga [Málaga] = University of Málaga [Málaga]
dc.contributor.authorCAÑAS, Rafael A
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLE PROVOST, Grégoire
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPLOMION, Christophe
hal.structure.identifierInstitut Technologique Forêt Cellulose Bois-construction Ameublement [FCBA]
dc.contributor.authorHARVENGT, Luc
hal.structure.identifierLaboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
dc.contributor.authorLABEL, Philippe
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorLELU-WALTER, Marie-Anne
hal.structure.identifierUniversidad de Málaga [Málaga] = University of Málaga [Málaga]
dc.contributor.authorCÁNOVAS, Francisco M
dc.date.issued2015
dc.date.conference2015-11-30
dc.description.abstractEnMaritime pine is a major forest tree in southern Europe for sustainable delivery of bio-based products through advanced plantation forestry of improved varieties. Early selection is needed to reduce breeding cycle and accelerate variety deployment in the context of climate change. Both gene(s) and genome-wide information should provide opportunities for predictive, marker-assisted selection. Reverse genetics, defined as ectopic expression or silencing of candidate genes can be useful for functional dissection of traits of interest. Functional genomics of 9 transcription factor (TF) genes associated to growth and wood formation (MYB1,8,14,20,23, DOF5, MADBOX4, NACx, NACataf) was initiated in maritime pine through genetic transformation of embryogenic tissue. Twelve TF constructs designed for constitutive overexpression (OE) or silencing (RNAi) were studied: 6 constructs (batch 1) from previous projects (MYB1-RNAi, MYB8-OE/RNAi, MYB14-RNAi, DOF5-OE/RNAi) as well as 3 constructs for putative gene targets of MYB (CAD-RNAi) and DOF5 (GS1a-OE, GS2-OE); and 6 constructs (batch 2) obtained during ProCoGen (MYB23-OE, MYB20-OE, MADBOX4-OE, NACx-OE/RNAi, NACataf-OE). Phosphinothricin-resistant lines could be cryopreserved for all but one (MYB20-OE) constructs. Transformation rate was estimated in the range 9.6-22.0 (OE) or 0.4-18.0 (RNAi) transgenic lines per gram embryogenic tissue (batch 1). Somatic embryos were obtained from 1-3 lines per construct (25 lines in total) and successfully converted to plants (batch 1). Acclimatization rates were similar to controls and transgenic plants were confirmed for most lines. Putative adverse effect of MYB14-RNAi, DOF5-RNAi, and MYB8-OE on transformation rate was observed. DOF5-RNAi apparently stimulated germination rate. Plant growth data and morphology are available for constructs from batch 1 after up to 12 (MYB1-RNAi, MYB8-OE/RNAi, DOF5-OE/RNAi, GS2-OE, GS1a-OE) or 42 months (MYB14-RNAi, CAD-RNAi) growth. Both transgene copy number and targeted gene expression data are available from transgenic plants obtained from MYB14-RNAi and CAD-RNAi lines. Transgenics and controls from batch 1 were sampled at age 16 (MYB1-RNAi, MYB8-OE/RNAi, DOF5-OE/RNAi) or 70 months (MYB14-RNAi, CAD-RNAi). Molecular characterization of MYB1,8,14 and DOF5 plant material has been performed by qPCR and transcriptomic analysis using microarray is underway. Wood analyses have been initiated for MYB14- and CAD-RNAi plants (in progress).
dc.language.isoen
dc.title.enTowards functional genomics of transcription factor genes associated to growth and wood formation in maritime pine
dc.typeAutre communication scientifique (congrès sans actes - poster - séminaire...)
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
dc.subject.halSciences du Vivant [q-bio]/Biologie du développement
bordeaux.page1
bordeaux.countryFR
bordeaux.conference.cityOrléans
bordeaux.peerReviewedoui
hal.identifierhal-02798385
hal.version1
hal.invitednon
hal.conference.end2015-12-02
hal.popularnon
hal.audienceNationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02798385v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2015&rft.spage=1&rft.epage=1&rft.au=TRONTIN,%20Jean-Fran%C3%A7ois&AVILA,%20Concepci%C3%B3n&DEBILLE,%20Sandrine&DE%20LA%20TORRE,%20Fernando&EL-AZAZ,%20Jorge&rft.genre=conference


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