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An easy and robust method for isolation and validation of single-nucleotide polymorphic markers from a first Erysiphe alphitoides draft genome

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorDUTECH, Cyril
hal.structure.identifierUniversity of British Columbia [UBC]
hal.structure.identifierDepartment of Forest and Conservation Sciences and Centre for Forest Conservation Genetics
dc.contributor.authorFEAU, N.
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierHelixVenture
dc.contributor.authorLESUR, Isabelle
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorEHRENMANN, François
hal.structure.identifierUnité de Recherche Génomique Info [URGI]
dc.contributor.authorLETELLIER, Thomas
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLI, B.
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorMOUDEN, Charlotte
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorDESPREZ-LOUSTAU, Marie Laure
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorGUICHOUX, Erwan
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierInstitut Fédéral de Recherches sur la Forêt, la Neige et le Paysage [WSL]
dc.contributor.authorGROSS, A.
dc.date.issued2020-06
dc.identifier.issn1617-416X
dc.description.abstractEnIsolating genetic markers is often costly and time-consuming for non-model fungal species. However, these markers are of primary importance to identify the origin of invasive species and to infer their reproductive mode and dispersal ability. We slightly modified a recent molecular method to quickly isolate and validate single-nucleotide polymorphism (SNP) markers, from a first Erysiphe alphitoides draft genome, one of the main causal agent of oak powdery mildew in Europe. Although the draft assembly was strongly fragmented (555,289 contigs), we successfully isolated 1700 SNPs from 75 single-copy genes conserved in most fungal genomes. Ninety percent of them allowed to clearly distinguish the two main Erysiphe species reported on European oaks: E. alphitoides and E. quercicola. Thirty-six SNPs, located in distinct genes, were then validated using a strategy of MassArray genotyping on 95 E. alphitoides isolates sampled in Europe. This genotyping showed that only monospore isolates had the expected haploid signature, whereas direct genotyping from field leaves showed signature of mixed infection. Considering haploid isolates, these markers led to the first results of population genetic diversity, and suggested that E. quercicola may have a more asexual reproduction than its sister species, E. alphitoides.
dc.language.isoen
dc.publisherSpringer Verlag
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectMarqueur génétique
dc.subjectErysiphe alphitoides
dc.subjectOidium
dc.subjectChene
dc.subjectErysiphe quercicola
dc.subject.enNucleotide Polymorphism
dc.subject.enQuercus
dc.subject.enBiological invasion
dc.subject.engenome assembly
dc.subject.enoak powdery mildew
dc.subject.enfungal forest pathogen
dc.subject.ensingle-copy genes
dc.subject.endevelopment of genetic markers
dc.title.enAn easy and robust method for isolation and validation of single-nucleotide polymorphic markers from a first Erysiphe alphitoides draft genome
dc.typeArticle de revue
dc.identifier.doi10.1007/s11557-020-01580-w
dc.subject.halSciences de l'environnement
dc.subject.halSciences de l'environnement/Biodiversité et Ecologie
dc.subject.halSciences de l'environnement/Milieux et Changements globaux
bordeaux.journalMycological Progress
bordeaux.page615-628
bordeaux.volume19
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-02768215
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02768215v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Mycological%20Progress&rft.date=2020-06&rft.volume=19&rft.issue=6&rft.spage=615-628&rft.epage=615-628&rft.eissn=1617-416X&rft.issn=1617-416X&rft.au=DUTECH,%20Cyril&FEAU,%20N.&LESUR,%20Isabelle&EHRENMANN,%20Fran%C3%A7ois&LETELLIER,%20Thomas&rft.genre=article


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