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Acute exposure to silica nanoparticles enhances mortality and increases lung permeability in a mouse model of Pseudomonas aeruginosa pneumonia.

hal.structure.identifierUnité de Biologie Fonctionnelle et Adaptative [BFA (UMR_8251 / U1133)]
dc.contributor.authorDELAVAL, Mathilde
hal.structure.identifierUnité de Biologie Fonctionnelle et Adaptative [BFA (UMR_8251 / U1133)]
dc.contributor.authorBOLAND, Sonja
hal.structure.identifierDéfense innée et inflammation
hal.structure.identifierPhysiopathologie et Epidémiologie des Maladies Respiratoires [PHERE (UMR_S_1152 / U1152)]
dc.contributor.authorSOLHONNE, Brigitte
hal.structure.identifierImagerie Dynamique (Plate-Forme) [PFID]
dc.contributor.authorNICOLA, Marie-Anne
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMORNET, Stéphane
hal.structure.identifierUnité de Biologie Fonctionnelle et Adaptative [BFA (UMR_8251 / U1133)]
dc.contributor.authorBAEZA-SQUIBAN, Armelle
hal.structure.identifierCellule Pasteur
hal.structure.identifierDéfense innée et inflammation
hal.structure.identifierPhysiopathologie et Epidémiologie des Maladies Respiratoires [PHERE (UMR_S_1152 / U1152)]
dc.contributor.authorSALLENAVE, Jean-Michel
hal.structure.identifierDéfense innée et inflammation
hal.structure.identifierPhysiopathologie et Epidémiologie des Maladies Respiratoires [PHERE (UMR_S_1152 / U1152)]
hal.structure.identifierCellule Pasteur
dc.contributor.authorGARCIA-VERDUGO, Ignacio
dc.date.issued2014-11-30
dc.identifier.issn1743-8977
dc.description.abstractEnThe lung epithelium constitutes the first barrier against invading pathogens and also a major surface potentially exposed to nanoparticles. In order to ensure and preserve lung epithelial barrier function, the alveolar compartment possesses local defence mechanisms that are able to control bacterial infection. For instance, alveolar macrophages are professional phagocytic cells that engulf bacteria and environmental contaminants (including nanoparticles) and secrete pro-inflammatory cytokines to effectively eliminate the invading bacteria/contaminants. The consequences of nanoparticle exposure in the context of lung infection have not been studied in detail. Previous reports have shown that sequential lung exposure to nanoparticles and bacteria may impair bacterial clearance resulting in increased lung bacterial loads, associated with a reduction in the phagocytic capacity of alveolar macrophages. Here we have studied the consequences of SiO2 nanoparticle exposure on Pseudomonas aeruginosa clearance, Pseudomonas aeruginosa-induced inflammation and lung injury in a mouse model of acute pneumonia. We observed that pre-exposure to SiO2 nanoparticles increased mice susceptibility to lethal pneumonia but did not modify lung clearance of a bioluminescent Pseudomonas aeruginosa strain. Furthermore, internalisation of SiO2 nanoparticles by primary alveolar macrophages did not reduce the capacity of the cells to clear Pseudomonas aeruginosa. In our murine model, SiO2 nanoparticle pre-exposure preferentially enhanced Pseudomonas aeruginosa-induced lung permeability (the latter assessed by the measurement of alveolar albumin and IgM concentrations) rather than contributing to Pseudomonas aeruginosa-induced lung inflammation (as measured by leukocyte recruitment and cytokine concentration in the alveolar compartment). We show that pre-exposure to SiO2 nanoparticles increases mice susceptibility to lethal pneumonia but independently of macrophage phagocytic function. The deleterious effects of SiO2 nanoparticle exposure during Pseudomonas aeruginosa-induced pneumonia are related to alterations of the alveolar-capillary barrier rather than to modulation of the inflammatory responses.
dc.language.isoen
dc.publisherBioMed Central
dc.subject.enAlveolar permeability
dc.subject.enInflammation
dc.subject.enInfection
dc.subject.enAlveolar macrophages
dc.subject.enSiO2
dc.subject.enNanoparticles
dc.subject.enPseudomonas
dc.subject.enLung
dc.subject.meshAnimals
dc.subject.meshBronchoalveolar Lavage Fluid
dc.subject.meshSurface Properties
dc.subject.meshSurvival Analysis
dc.subject.meshCapillary Permeability
dc.subject.meshCytokines
dc.subject.meshImmunoglobulin M
dc.subject.meshInhalation Exposure
dc.subject.meshMacrophages, Alveolar
dc.subject.meshMale
dc.subject.meshMice, Inbred C57BL
dc.subject.meshNanoparticles
dc.subject.meshParticle Size
dc.subject.meshPhagocytosis
dc.subject.meshPneumonia, Bacterial
dc.subject.meshPseudomonas Infections
dc.subject.meshPseudomonas aeruginosa
dc.subject.meshPulmonary Alveoli
dc.subject.meshSelenium Oxides
dc.title.enAcute exposure to silica nanoparticles enhances mortality and increases lung permeability in a mouse model of Pseudomonas aeruginosa pneumonia.
dc.typeArticle de revue
dc.identifier.doi10.1186/s12989-014-0078-9
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalParticle and Fibre Toxicology
bordeaux.page1
bordeaux.volume12
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierinserm-01264515
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-01264515v1
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