Afficher la notice abrégée

Interaction of freshwater diatom with gold nanoparticles: adsorption, assimilation, and stabilization by cell exometabolites

hal.structure.identifierInstituto de Oceanografía y Cambio Global [IOCAG]
hal.structure.identifierGéosciences Environnement Toulouse [GET]
dc.contributor.authorGONZALEZ, Aridane G.
hal.structure.identifierGéosciences Environnement Toulouse [GET]
hal.structure.identifierTomsk State University [Tomsk]
dc.contributor.authorPOKROVSKY, Oleg
hal.structure.identifierN. Laverov Federal Center for Integrated Arctic Research of the Ural Branch [FECIAR UrB RAS ]
hal.structure.identifier​Trofimuk Institute of Petroleum Geology and Geophysics of the Siberian Branch of the RAS [IPGG SB RAS]
dc.contributor.authorIVANOVA, Irina S.
hal.structure.identifierGéosciences Environnement Toulouse [GET]
dc.contributor.authorOLEINIKOVA, Olga
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorFEURTET-MAZEL, Agnès
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMORNET, Stéphane
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBAUDRIMONT, Magalie
dc.date.issued2018
dc.identifier.issn2075-163X
dc.description.abstractEnThe rising concern about the potential toxicity of synthetic gold nanoparticles (AuNPs) in aquatic environments requires a rigorous estimation of physico-chemical parameters of reactions between AuNPs and major freshwater microorganisms. This study addresses the interaction of 10-nm size, positively charged AuNPs with periphytic freshwater diatoms (Eolimna minima). The adsorption experiments on viable cells were performed in 10 mM NaCl and 5 mM NaCl + 5 mM NaHCO3 solution at a variable pH (3–10), at an AuNPs concentration from 1 µg/L to 10,000 µg/L, and an exposure time from a few minutes to 55 days. Three types of experiments, adsorption as a function of time (kinetics), pH-dependent adsorption edge, and constant-pH “Langmuirian” type isotherms, were conducted. In addition, long-term interactions (days to weeks) of live diatoms (under light and in the darkness) were performed. The adsorption was maximal at a pH from 3 to 6 and sizably decreased at a pH of 6 to 10. Results of adsorption experiments were modeled using a second order kinetic model, a Linear Programming Model, Freundlich isotherm, and a ligand binding equation for one site competition. The adsorption of AuNPs(+) most likely occurred on negatively-charged surface sites of diatom cell walls such as carboxylates or phosphorylates, similar to previously studied metal cations. Under light exposure, the AuNPs were stabilized in aqueous solution in the presence of live cells, probably due to the production of exometabolites by diatoms. The adsorbed amount of AuNPs decreased after several days of reaction, suggesting some AuNPs desorption. In the darkness, the adsorption and assimilation were stronger than under light. Overall, the behavior of positively charged AuNPs at the diatom–aqueous solution interface is similar to that of metal cations, but the affinity of aqueous AuNPs to cell exometabolites is higher, which leads to the stabilization of nanoparticles in solution in the presence of diatoms and their exudates. During photosynthetic activity and the pH rising above 9 in the vicinity of diatom cells, the adsorption of AuNPs strongly decreases, which indicates a decreasing potential toxicity of AuNPs for photosynthesizing cells. The present study demonstrates the efficiency of a thermodynamic and kinetic approach for understanding gold nanoparticles interaction with aquatic freshwater peryphytic microorganisms.
dc.description.sponsorshipApproches à différentes échelles pour caractériser les interactions cellulaires, le transfert trophique et les impacts toxiques de nanoparticules métalliques chez les organismes aquatiques - ANR-14-CE21-0001
dc.language.isoen
dc.publisherMDPI
dc.subject.enAuNPs
dc.subject.enfreshwater diatoms
dc.subject.enbiofilm
dc.subject.enadsorption
dc.subject.enriver
dc.subject.enpollution
dc.title.enInteraction of freshwater diatom with gold nanoparticles: adsorption, assimilation, and stabilization by cell exometabolites
dc.typeArticle de revue
dc.identifier.doi10.3390/min8030099
dc.subject.halSciences du Vivant [q-bio]/Toxicologie/Ecotoxicologie
dc.subject.halChimie/Matériaux
bordeaux.journalMinerals
bordeaux.page99
bordeaux.volume8
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-01799939
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01799939v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Minerals&rft.date=2018&rft.volume=8&rft.issue=3&rft.spage=99&rft.epage=99&rft.eissn=2075-163X&rft.issn=2075-163X&rft.au=GONZALEZ,%20Aridane%20G.&POKROVSKY,%20Oleg&IVANOVA,%20Irina%20S.&OLEINIKOVA,%20Olga&FEURTET-MAZEL,%20Agn%C3%A8s&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée