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hal.structure.identifierInstitut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux [IPREM]
dc.contributor.authorARNAUDGUILHEM, Carine
hal.structure.identifierInstitut régional de Cancérologie de Montpellier [ICM]
hal.structure.identifierInstitut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux [IPREM]
dc.contributor.authorLARROQUE, Marion
hal.structure.identifierInstitut régional de Cancérologie de Montpellier [ICM]
hal.structure.identifierDépartement de chirurgie
dc.contributor.authorSGARBURA, Olivia
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMICHAU, Dominique
hal.structure.identifierInstitut régional de Cancérologie de Montpellier [ICM]
hal.structure.identifierDépartement de chirurgie
dc.contributor.authorQUENET, François
hal.structure.identifierInstitut régional de Cancérologie de Montpellier [ICM]
hal.structure.identifierDépartement de chirurgie
dc.contributor.authorCARRERE, Sébastien
hal.structure.identifierInstitut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux [IPREM]
dc.contributor.authorBOUYSSIÈRE, Brice
hal.structure.identifierInstitut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux [IPREM]
dc.contributor.authorMOUNICOU, Sandra
dc.date.issued2020-08-14
dc.identifier.issn0039-9140
dc.description.abstractEnQuantitative localization of metals in biological tissue sections is critical to obtain insight into metal toxicity mechanisms or their beneficial characteristics. This study presents the development of a quantitative LA-ICP MS bioimaging methodology based on the polymer film strategy and internal standardization. To maximize the number of elements mapped, an aqueous soluble polymer (dextran) was selected. Among the elements studied, the great majority (eight out eleven), i.e., Co, Ni, Cu, Zn, Se, Mo, Cd and Pt, exhibited linear regression after LA-ICP MS analysis of metal-spiked polymer standards. Methodology performances were carefully assessed as a function of the three internal standards (In, Rh and Ir) considered, the analytical operational conditions (ICP power, addition of O 2 to ICP, and laser fluency) and the thickness of the biological tissue section. The results indicated that three groups (Co, Mo; Ni, Cu, Pt; and Zn, Se, Cd) of elements could be distinguished from their analytical response as a function of analytical conditions and the internal standard. These different element behaviors appeared to be mainly First Ionization Potential dependent (FIP). For elements with lower FIP (Co, Ni, Cu, Mo and Pt), differential responses due to carbon load in the ICP MS plasma could be efficiently corrected as a function of analytical conditions. Matrix effects were more pronounced for higher FIP elements (i.e., Zn, Cd and Se), and analysis of <10-μm thin sections without the addition of O 2 to ICP MS plasma is recommended. LODs are in the range of 0.1-0.5 μg g − 1 for Co, Mo, Cu, Ni, Pt and Cd as well as 0.9 and 1 μg g − 1 for Zn and Se, respectively. The methodology was validated by means of a homemade metal-spiked kidney homogenate analyzed by LA-ICP MS imaging, and Co, Ni, Cu, Mo, and Pt provided the closest concentrations (5-29% bias) to the target values determined by ICP MS after mineralization. The methodology was applied to two types of clinical human samples undergoing different sample preparation protocols that did not affect internal standard homogeneity in the polymer film. This methodology is the first reported for the quantitative bioimaging of eight elements simultaneously.
dc.language.isoen
dc.publisherElsevier
dc.subject.enLA-ICP MS
dc.subject.enbioimaging
dc.subject.enquantification
dc.subject.enmultielemental
dc.subject.enpolymer films
dc.title.enToward a comprehensive study for multielemental quantitative LA-ICP MS bioimaging in soft tissues
dc.typeArticle de revue
dc.identifier.doi10.1016/j.talanta.2020.121537
dc.subject.halChimie/Chimie analytique
bordeaux.journalTalanta
bordeaux.page121537 (11 p.)
bordeaux.volume222
bordeaux.peerReviewedoui
hal.identifierhal-03019255
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03019255v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Talanta&amp;rft.date=2020-08-14&amp;rft.volume=222&amp;rft.spage=121537%20(11%20p.)&amp;rft.epage=121537%20(11%20p.)&amp;rft.eissn=0039-9140&amp;rft.issn=0039-9140&amp;rft.au=ARNAUDGUILHEM,%20Carine&amp;LARROQUE,%20Marion&amp;SGARBURA,%20Olivia&amp;MICHAU,%20Dominique&amp;QUENET,%20Fran%C3%A7ois&amp;rft.genre=article


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