Internalization and fate of silica nanoparticles in C2C12 skeletal muscle cells: evidence of a beneficial effect on myoblast fusion.
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | POUSSARD, Sylvie | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | DECOSSAS, Marion | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | LE BIHAN, Olivier | |
| hal.structure.identifier | Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB] | |
| dc.contributor.author | MORNET, Stéphane | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | NAUDIN, Grégoire | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | LAMBERT, Olivier | |
| dc.date.issued | 2015 | |
| dc.identifier.issn | 1176-9114 | |
| dc.description.abstractEn | The use of silica nanoparticles for their cellular uptake capability opens up new fields in biomedical research. Among the toxicological effects associated with their internalization, silica nanoparticles induce apoptosis that has been recently reported as a biochemical cue required for muscle regeneration. To assess whether silica nanoparticles could affect muscle regeneration, we used the C2C12 muscle cell line to study the uptake of fluorescently labeled NPs and their cellular trafficking over a long period. Using inhibitors of endocytosis, we determined that the NP uptake was an energy-dependent process mainly involving macropinocytosis and clathrin-mediated pathway. NPs were eventually clustered in lysosomal structures. Myoblasts containing NPs were capable of differentiation into myotubes, and after 7 days, electron microscopy revealed that the NPs remained primarily within lysosomes. The presence of NPs stimulated the formation of myotubes in a dose-dependent manner. NP internalization induced an increase of apoptotic myoblasts required for myoblast fusion. At noncytotoxic doses, the NP uptake by skeletal muscle cells did not prevent their differentiation into myotubes but, instead, enhanced the cell fusion. | |
| dc.language.iso | en | |
| dc.publisher | Dove Medical Press | |
| dc.subject.en | silica | |
| dc.subject.en | nanoparticle | |
| dc.subject.en | muscle | |
| dc.subject.en | cell encapsulation | |
| dc.subject.en | transmission electron microscopy | |
| dc.subject.en | apoptosis | |
| dc.title.en | Internalization and fate of silica nanoparticles in C2C12 skeletal muscle cells: evidence of a beneficial effect on myoblast fusion. | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.2147/IJN.S74158 | |
| dc.subject.hal | Chimie/Matériaux | |
| bordeaux.journal | International Journal of Nanomedicine | |
| bordeaux.page | 1479-1492 | |
| bordeaux.volume | 10 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-01128811 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-01128811v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=International%20Journal%20of%20Nanomedicine&rft.date=2015&rft.volume=10&rft.spage=1479-1492&rft.epage=1479-1492&rft.eissn=1176-9114&rft.issn=1176-9114&rft.au=POUSSARD,%20Sylvie&DECOSSAS,%20Marion&LE%20BIHAN,%20Olivier&MORNET,%20St%C3%A9phane&NAUDIN,%20Gr%C3%A9goire&rft.genre=article |
Fichier(s) constituant ce document
| Fichiers | Taille | Format | Vue |
|---|---|---|---|
|
Il n'y a pas de fichiers associés à ce document. |
|||