Mostrar el registro sencillo del ítem

The effect of RGD density on osteoblast and endothelial cell behavior on RGD-grafted polyethylene terephthalate surfaces

hal.structure.identifierBiomateriaux et Reparation Tissulaire
dc.contributor.authorCHOLLET, Céline
hal.structure.identifierBiomateriaux et Reparation Tissulaire
dc.contributor.authorCHANSEAU, Christel
hal.structure.identifierBiomateriaux et Reparation Tissulaire
dc.contributor.authorRÉMY, Murielle
hal.structure.identifierContraintes mécaniques et tissu osseux
dc.contributor.authorGUIGNANDON, Alain
hal.structure.identifierBiomateriaux et Reparation Tissulaire
dc.contributor.authorBAREILLE, Reine
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorLABRUGÈRE, Christine
hal.structure.identifierBiomateriaux et Reparation Tissulaire
hal.structure.identifierImmunologie antivirale systémique et cérébrale
dc.contributor.authorBORDENAVE, Laurence
hal.structure.identifierBiomateriaux et Reparation Tissulaire
dc.contributor.authorDURRIEU, Marie-Christine
dc.date.issued2009-02
dc.identifier.issn0142-9612
dc.description.abstractEnHybrid materials combining polyethylene terephthalate and different types of cells (endothelial and osteoblastic cells) have been developed thanks to the covalent grafting of different densities of RGD containing peptides onto the polymer surface. Biomimetic modifications were performed by means of a three-step reaction procedure: creation of COOH functions, coupling agent grafting and the immobilization of the RGDC peptides. High resolution μ-imager was used to evaluate RGD densities (varying between 0.6 and 2.4 pmol/mm2) and has exhibited the stability of the surface grafted peptides when treated in harsh conditions. The efficiency of this route for biomimetic modification of a PET surface was demonstrated by measuring the adhesion of MC3T3 and HSVEC cells and by focal adhesion observation. Results obtained prove that a minimal RGDC density of 1 pmol/mm2 is required to improve MC3T3 and HSVEC cells responses. Indeed, cells seeded onto a RGDC-modified PET with a density higher than 1 pmol/mm2 were able to establish focal adhesion as visualized by fluorescence microscope compared to cells immobilized onto unmodified PET and RGDC-modified PET with densities lower than 1 pmol/mm2. Moreover, the number of focal contacts was enhanced by the increase of RGDC peptide densities grafted onto the material surface. With this study we proved that the density of peptides immobilized on the surface is a very important parameter influencing osteoblast or endothelial cell adhesion and focal contact formation.
dc.language.isoen
dc.publisherElsevier
dc.subject.enSurface modification
dc.subject.enRGD peptides
dc.subject.enEndothelial cells
dc.subject.enOsteoblast
dc.subject.enFocal adhesion
dc.subject.meshAnimals
dc.subject.meshBiocompatible Materials
dc.subject.meshCell Adhesion
dc.subject.meshCell Line
dc.subject.meshEndothelial Cells
dc.subject.meshMice
dc.subject.meshOligopeptides
dc.subject.meshOsteoblasts
dc.subject.meshPolyethylene Terephthalates
dc.subject.meshSurface Properties
dc.title.enThe effect of RGD density on osteoblast and endothelial cell behavior on RGD-grafted polyethylene terephthalate surfaces
dc.typeArticle de revue
dc.identifier.doi10.1016/j.biomaterials.2008.10.033
dc.subject.halChimie/Matériaux
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
bordeaux.journalBiomaterials
bordeaux.page711-720
bordeaux.volume30
bordeaux.issue5
bordeaux.peerReviewedoui
hal.identifierhal-00351437
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00351437v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomaterials&rft.date=2009-02&rft.volume=30&rft.issue=5&rft.spage=711-720&rft.epage=711-720&rft.eissn=0142-9612&rft.issn=0142-9612&rft.au=CHOLLET,%20C%C3%A9line&CHANSEAU,%20Christel&R%C3%89MY,%20Murielle&GUIGNANDON,%20Alain&BAREILLE,%20Reine&rft.genre=article


Archivos en el ítem

ArchivosTamañoFormatoVer

No hay archivos asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem