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hal.structure.identifierLaboratoire de Biologie cellulaire
dc.contributor.authorL'AZOU, Béatrice
hal.structure.identifierLaboratoire de Biologie cellulaire
dc.contributor.authorPASSAGNE, Isabelle
hal.structure.identifierInstitut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux [IPREM]
dc.contributor.authorMOUNICOU, Sandra
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorTRÉGUER-DELAPIERRE, Mona
hal.structure.identifierLaboratoire de Biologie cellulaire
dc.contributor.authorPUJALTÉ, Igor
hal.structure.identifierInstitut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux [IPREM]
dc.contributor.authorSZPUNAR, Joanna
hal.structure.identifierInstitut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux [IPREM]
dc.contributor.authorLOBINSKI, Ryszard
hal.structure.identifierLaboratoire Hydrologie-Environnement
dc.contributor.authorOHAYON-COURTÈS, Céline
dc.date.issued2014
dc.identifier.issn2045-452X
dc.description.abstractEnCadmium (Cd) is a potent selective toxicant that preferentially accumulates in the kidneys where it is known to induce cellular injury. The cellular mechanisms explaining toxicity have been well documented by in vivo and in vitro studies. However, experiments have mainly been performed on cadmium chloride (CdCl2), while several other forms, such as cadmium oxide (CdO) and cadmium sulfide (CdS), are of interest regarding occupational exposure. CdS is mainly used in nanotechnology, primarily to construct particles known as quantum dots (QDs). The present study focuses on cadmium forms that differ compared to their solubility and their particle size (CdS micro- and uncoated nanoparticles (NPs)), and investigates their effects on mesangial and proximal tubular kidney cells. Cellular toxicity was evaluated by using Neutral Red, MTT and WST-1 assays. Intracellular Cd content was measured using inductively coupled optical emission spectrometry (ICP-OES) and cellular stress was investigated via the effects of Cd complexing bioligands using size exclusion chromatography ICP-MS (SEC-ICP-MS). Data indicated the variability of cytotoxicity responses after CdCl2, CdO, and CdS exposure that were strongly correlated to the cellular Cd content. Release of Cd2+ linked to solubility and particle degradation were considered to be the cause of these toxicities. The bioinduction of the Cd-MT also evidenced release of Cd2+. Our in vitro data identified heterogeneity of Cd toxicity that was dependent on the physico-chemical properties of the form studied and, when considering particle size, constitute an additional step toward the determination of nanoparticle effects in renal cells.
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.subject.enCytotoxicity
dc.subject.enCadmium forms
dc.subject.enRenal cells
dc.title.enComparative cytotoxicity of cadmium forms (CdCl2, CdO, CdS micro- and nanoparticles) in renal cells
dc.typeArticle de revue
dc.identifier.doi10.1039/c3tx50063b
dc.subject.halChimie/Matériaux
bordeaux.journalToxicology Research
bordeaux.page32-41
bordeaux.volume3
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-00923649
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00923649v1
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