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Cell Confluence Modulates TRPV4 Channel Activity in Response to Hypoxia

dc.rights.licenseopenen_US
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorBARBEAU, Solene
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorJOUSHOMME, Alexandre
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorCHAPPE, Yann
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorCARDOUAT, Guillaume
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorBAUDRIMONT, Isabelle
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorFREUND-MICHEL, Véronique
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorGUIBERT, Christelle
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorMARTHAN, Roger
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorBERGER, Patrick
ORCID: 0000-0003-4702-0343
IDREF: 060717998
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorVACHER, Pierre
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorPERCHERANCIER, Yann
IDREF: 075934140
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorQUIGNARD, Jean-François
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorDUCRET, Thomas
dc.date.accessioned2022-08-29T12:31:08Z
dc.date.available2022-08-29T12:31:08Z
dc.date.issued2022-07-07
dc.identifier.issn2218-273Xen_US
dc.identifier.otherhttps://www.mdpi.com/2218-273X/12/7/954/s1?version=1657173560en_US
dc.identifier.urioai:crossref.org:10.3390/biom12070954
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/140612
dc.description.abstractEnTransient receptor potential vanilloid 4 (TRPV4) is a polymodal Ca2+-permeable channel involved in various hypoxia-sensitive pathophysiological phenomena. Different tools are available to study channel activity, requiring cells to be cultured at specific optimal densities. In the present study, we examined if cell density may influence the effect of hypoxia on TRPV4 activity. Transiently TRPV4-transfected HEK293T cells were seeded at low or high densities corresponding to non-confluent or confluent cells, respectively, on the day of experiments, and cultured under in vitro normoxia or hypoxia. TRPV4-mediated cytosolic Ca2+ responses, single-channel currents, and Ca2+ influx through the channel were measured using Ca2+ imaging/microspectrofluorimetric assay, patch-clamp, and Bioluminescence Resonance Energy Transfer (BRET), respectively. TRPV4 plasma membrane translocation was studied using confocal microscopy, biotinylation of cell surface proteins, and BRET. Our results show that hypoxia exposure has a differential effect on TRPV4 activation depending on cell confluence. At low confluence levels, TRPV4 response is increased in hypoxia, whereas at high confluence levels, TRPV4 response is strongly inhibited, due to channel internalization. Thus, cell density appears to be a crucial parameter for TRPV4 channel activity.
dc.description.sponsorshipDépasser les limites des méthodes existantes de criblage à haut débit et de caractérisation des canaux ioniques grâce au transfert d'énergie en résonance de bioluminescence - ANR-19-CE44-0010en_US
dc.language.isoENen_US
dc.sourcecrossref
dc.subject.enBRET
dc.subject.encalcium
dc.subject.encell culture conditions
dc.subject.encell density
dc.subject.enpatch-clamp
dc.subject.enstretch-activated channel
dc.subject.enTRP channel
dc.title.enCell Confluence Modulates TRPV4 Channel Activity in Response to Hypoxia
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/biom12070954en_US
dc.subject.halSciences de l'ingénieur [physics]/Traitement du signal et de l'imageen_US
bordeaux.journalBiomoleculesen_US
bordeaux.page954en_US
bordeaux.volume12en_US
bordeaux.hal.laboratoriesLaboratoire d’Intégration du Matériau au Système (IMS) - UMR 5218en_US
bordeaux.issue7en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.institutionINSERM
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-03763361
hal.version1
hal.date.transferred2022-08-29T12:31:11Z
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomolecules&rft.date=2022-07-07&rft.volume=12&rft.issue=7&rft.spage=954&rft.epage=954&rft.eissn=2218-273X&rft.issn=2218-273X&rft.au=BARBEAU,%20Solene&JOUSHOMME,%20Alexandre&CHAPPE,%20Yann&CARDOUAT,%20Guillaume&BAUDRIMONT,%20Isabelle&rft.genre=article


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