Mostrar el registro sencillo del ítem

Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray micro-spectroscopy

hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorORTEGA, R.
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorDEVÈS, Guillaume
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorCARMONA, A.
dc.date.issued2009
dc.identifier.issn1742-5689
dc.description.abstractEnThe direct detection of biologically relevant metals in single cells and of their speciation is a challenging task that requires sophisticated analytical developments. The aim of this article is to present the recent achievements in the field of cellular chemical element imaging, and direct speciation analysis, using proton and synchrotron radiation X-ray micro- and nano-analysis. The recent improvements in focusing optics for MeV-accelerated particles and keV X-rays allow application to chemical element analysis in subcellular compartments. The imaging and quantification of trace elements in single cells can be obtained using particle-induced X-ray emission (PIXE). The combination of PIXE with backscattering spectrometry and scanning transmission ion microscopy provides a high accuracy in elemental quantification of cellular organelles. On the other hand, synchrotron radiation X-ray fluorescence provides chemical element imaging with less than 100 nm spatial resolution. Moreover, synchrotron radiation offers the unique capability of spatially resolved chemical speciation using micro-X-ray absorption spectroscopy. The potential of these methods in biomedical investigations will be illustrated with examples of application in the fields of cellular toxicology, and pharmacology, bio-metals and metal-based nano-particles.
dc.language.isoen
dc.publisherthe Royal Society
dc.title.enBio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray micro-spectroscopy
dc.typeArticle de revue
dc.identifier.doi10.1098/rsif.2009.0166.focus
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.journalJournal of the Royal Society Interface
bordeaux.pageS649-S658
bordeaux.volume6
bordeaux.peerReviewedoui
hal.identifierin2p3-00762347
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//in2p3-00762347v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20the%20Royal%20Society%20Interface&rft.date=2009&rft.volume=6&rft.spage=S649-S658&rft.epage=S649-S658&rft.eissn=1742-5689&rft.issn=1742-5689&rft.au=ORTEGA,%20R.&DEV%C3%88S,%20Guillaume&CARMONA,%20A.&rft.genre=article


Archivos en el ítem

ArchivosTamañoFormatoVer

No hay archivos asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem