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Fluorescence time-lapse imaging of single cells targeted with a focused scanning charged-particle microbeam

hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorBOURRET, S.
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorVIANNA, F.
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorDEVÈS, Guillaume
dc.contributor.authorATALLAH, V.
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorMORETTO, P.
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorSEZNEC, H.
hal.structure.identifierInterface Physique et Chimie pour le Vivant [IPCV]
dc.contributor.authorBARBERET, P.
dc.date.issued2014
dc.identifier.issn0168-583X
dc.description.abstractEnCharged particle microbeams provide unique features to study targeted and non-targeted radiation response and have recently emerged as a powerful tool to investigate radiation-induced DNA damage and repair. We have developed a charged particle microbeam delivering protons and alpha particles in the MeV energy range equipped with online time-lapse imaging capabilities. The beam is focused to a sub-micrometer beam spot under vacuum by means of a triplet of magnetic quadrupoles and extracted in air through a 200 nm Si3N4 window. The end-station is equipped with an automated fluorescence microscope used for single cell targeting and online time-lapse imaging. Cells are kept in their medium during the irradiation procedure and the sample temperature is regulated to 37 C. An overall targeting accuracy of 2.0 ± 0.7 lm has been measured by tracking the re-localization of the XRCC1 protein. First measurements of this re-localization shows the ability of our system to follow online the radiationinduced re-localization of proteins in the first minutes after irradiation.
dc.language.isoen
dc.publisherElsevier
dc.subject.enMicrobeam Single cell Time-lapse microscopy Targeted irradiation Radiation biology
dc.title.enFluorescence time-lapse imaging of single cells targeted with a focused scanning charged-particle microbeam
dc.typeArticle de revue
dc.identifier.doi10.1016/j.nimb.2014.02.004
dc.subject.halPhysique [physics]/Physique [physics]/Instrumentations et Détecteurs [physics.ins-det]
bordeaux.journalNuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
bordeaux.page27-34
bordeaux.volume325
bordeaux.peerReviewedoui
hal.identifierin2p3-00956455
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//in2p3-00956455v1
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