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Live cell imaging of mitochondria following targeted irradiation in situ reveals rapid and highly localized loss of membrane potential

dc.contributor.authorWALSH, Dietrich W. M.
dc.contributor.authorSIEBENWIRTH, Christian
dc.contributor.authorGREUBEL, Christoph
dc.contributor.authorILICIC, Katarina
dc.contributor.authorREINDL, Judith
dc.contributor.authorGIRST, Stefanie
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorMUGGIOLU, Giovanna
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorSIMON, Marina
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorBARBERET, Philippe
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorSEZNEC, Hervé
dc.contributor.authorZISCHKA, Hans
dc.contributor.authorMULTHOFF, Gabriele
dc.contributor.authorSCHMID, Thomas E.
dc.contributor.authorDOLLINGER, Guenther
dc.date.issued2017
dc.identifier.issn2045-2322
dc.description.abstractEnThe reliance of all cell types on the mitochondrial function for survival makes mitochondria an interesting target when trying to understand their role in the cellular response to ionizing radiation. By harnessing highly focused carbon ions and protons using microbeams, we have performed in situ live cell imaging of the targeted irradiation of individual mitochondria stained with Tetramethyl rhodamine ethyl ester (TMRE), a cationic fluorophore which accumulates electrophoretically in polarized mitochondria. Targeted irradiation with both carbon ions and protons down to beam spots of <1 μm induced a near instant loss of mitochondrial TMRE fluorescence signal in the targeted area. The loss of TMRE after targeted irradiation represents a radiation induced change in mitochondrial membrane potential. This is the first time such mitochondrial responses have been documented in situ after targeted microbeam irradiation. The methods developed and the results obtained have the ability to shed new light on not just mitochondria’s response to radiation but to further elucidate a putative mechanism of radiation induced depolarization and mitochondrial response.
dc.language.isoen
dc.publisherNature Publishing Group
dc.title.enLive cell imaging of mitochondria following targeted irradiation in situ reveals rapid and highly localized loss of membrane potential
dc.typeArticle de revue
dc.identifier.doi10.1038/srep46684
dc.subject.halPhysique [physics]
bordeaux.journalScientific Reports
bordeaux.page46684
bordeaux.volume7
bordeaux.peerReviewedoui
hal.identifierhal-01763837
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01763837v1
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