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Isotopic variations of copper at the protein level in neuronal human cells exposed in vitro to uranium

hal.structure.identifierCEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) [CEA-DES (ex-DEN)]
dc.contributor.authorPAREDES, Eduardo
hal.structure.identifierInstitut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
hal.structure.identifierInteractions Protéine Métal [IPM]
dc.contributor.authorMALARD, Veronique
hal.structure.identifierInstitut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
dc.contributor.authorVIDAUD, Claude
hal.structure.identifierCEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) [CEA-DES (ex-DEN)]
dc.contributor.authorAVAZERI, Emilie
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorORTEGA, Richard
hal.structure.identifierLaboratoire de développement Analytique Nucléaire Isotopique et Elémentaire [LANIE]
dc.contributor.authorNONELL, Anthony
hal.structure.identifierLaboratoire de développement Analytique Nucléaire Isotopique et Elémentaire [LANIE]
dc.contributor.authorISNARD, Hélène
hal.structure.identifierDépartement de Physico-Chimie [DPC]
dc.contributor.authorCHARTIER, Frédéric
hal.structure.identifierLaboratoire de développement Analytique Nucléaire Isotopique et Elémentaire [LANIE]
dc.contributor.authorBRESSON, Carole
dc.date.issued2019-08-30
dc.identifier.issn0003-2654
dc.description.abstractEnThe study of isotopic variations of endogenous and toxic metals in fluids and tissues is a recent research topic with an outstanding potential in biomedical and toxicological investigations. Most of the analyses have been performed so far in bulk samples, which can make the interpretation of results entangled, since different sources of stress or the alteration of different metabolic processes can lead to similar variations in the isotopic compositions of the elements in bulk samples. The downscaling of the isotopic analysis of elements at the sub-cellular level, is considered as a more promising alternative. Here we present for the first time the accurate determination of Cu isotopic ratios in four main protein fractions from lysates of neuron-like human cells exposed in vitro to 10 mu M of natural uranium for seven days. These protein fractions were isolated by Size Exclusion Chromatography and analysed by Multi-Collector Inductively Coupled Plasma Mass Spectrometry to determine the Cu isotopic variations in each protein fraction with regard to the original cell lysate. Values obtained, expressed as delta Cu-65, were -0.03 +/- 0.14 % (U-c, k = 2), -0.55 +/- 0.20 % (U-c, k = 2), -0.32 +/- 0.21 % (U-c, k = 2) and + 0.84 +/- 0.21 % (U-c, k = 2) for the four fractions, satisfying the mass balance. The results obtained in this preliminary study pave the way for dedicated analytical developments to identify new specific disease biomarkers, to gain insight into stress-induced altered metabolic processes, as well as to decipher metabolic pathways of toxic elements.
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.title.enIsotopic variations of copper at the protein level in neuronal human cells exposed in vitro to uranium
dc.typeArticle de revue
dc.identifier.doi10.1039/C9AN01081E
dc.subject.halSciences du Vivant [q-bio]
dc.description.sponsorshipEuropeEnhanced Eurotalents: a European programme for transnational mobility of experimented researchers managed by CEA
bordeaux.journalAnalyst
bordeaux.page5928-5933
bordeaux.volume144
bordeaux.issue20
bordeaux.peerReviewedoui
hal.identifierhal-02447119
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02447119v1
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