Full-length Dhh and N-terminal Shh act as competitive antagonists to regulate angiogenesis and vascular permeability
| dc.rights.license | open | en_US |
| hal.structure.identifier | Biologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases | |
| dc.contributor.author | HOLLIER, Pierre-Louis | |
| hal.structure.identifier | Biologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases | |
| dc.contributor.author | CHAPOULY, Candice | |
| hal.structure.identifier | Biologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases | |
| dc.contributor.author | DIOP, Aissata | |
| hal.structure.identifier | Biologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases | |
| dc.contributor.author | GUIMBAL, Sarah | |
| hal.structure.identifier | Biologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases | |
| dc.contributor.author | CORNUAULT, Lauriane | |
| hal.structure.identifier | Biologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases | |
| dc.contributor.author | GADEAU, Alain-Pierre | |
| hal.structure.identifier | Biologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases | |
| dc.contributor.author | RENAULT, Marie-Ange | |
| dc.date.accessioned | 2022-02-03T16:16:06Z | |
| dc.date.available | 2022-02-03T16:16:06Z | |
| dc.date.issued | 2021-11-01 | |
| dc.identifier.issn | 1755-3245 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/124664 | |
| dc.description.abstractEn | The therapeutic potential of Hedgehog (Hh) signalling agonists for vascular diseases is of growing interest. However, molecular and cellular mechanisms underlying the role of the Hh signalling in vascular biology remain poorly understood. The purpose of the present article is to clarify some conflicting literature data. With this goal, we have demonstrated that, unexpectedly, ectopically administered N-terminal Sonic Hh (N-Shh) and endogenous endothelial-derived Desert Hh (Dhh) induce opposite effects in endothelial cells (ECs). Notably, endothelial Dhh acts under its full-length soluble form (FL-Dhh) and activates Smoothened in ECs, while N-Shh inhibits it. At molecular level, N-Shh prevents FL-Dhh binding to Patched-1 (Ptch1) demonstrating that N-Shh acts as competitive antagonist to FL-Dhh. Besides, we found that even though FL-Hh ligands and N-Hh ligands all bind Ptch1, they induce distinct Ptch1 localization. Finally, we confirmed that in a pathophysiological setting, i.e. brain inflammation, astrocyte-derived N-Shh acts as a FL-Dhh antagonist. The present study highlights for the first time that FL-Dhh and N-Hh ligands have antagonistic properties especially in ECs. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Animals | |
| dc.subject.en | Astrocytes | |
| dc.subject.en | Capillary Permeability | |
| dc.subject.en | Cells | |
| dc.subject.en | Cultured | |
| dc.subject.en | Cerebral Cortex | |
| dc.subject.en | Corneal Neovascularization | |
| dc.subject.en | Encephalomyelitis | |
| dc.subject.en | Autoimmune | |
| dc.subject.en | Experimental | |
| dc.subject.en | Endothelial Cells | |
| dc.subject.en | Female | |
| dc.subject.en | Hedgehog Proteins | |
| dc.subject.en | Ligands | |
| dc.subject.en | Male | |
| dc.subject.en | Mice | |
| dc.subject.en | Knockout | |
| dc.subject.en | Neovascularization | |
| dc.subject.en | Pathologic | |
| dc.subject.en | Patched-1 Receptor | |
| dc.subject.en | Protein Binding | |
| dc.subject.en | Signal Transduction | |
| dc.subject.en | Smoothened Receptor | |
| dc.title.en | Full-length Dhh and N-terminal Shh act as competitive antagonists to regulate angiogenesis and vascular permeability | |
| dc.title.alternative | Cardiovasc Res | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1093/cvr/cvaa285 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Cardiologie et système cardiovasculaire | en_US |
| dc.identifier.pubmed | 33063110 | en_US |
| bordeaux.journal | Cardiovascular Research | en_US |
| bordeaux.page | 2489-2501 | en_US |
| bordeaux.volume | 117 | en_US |
| bordeaux.hal.laboratories | Biologie des maladies cardiovasculaires (BMC) - UMR 1034 | en_US |
| bordeaux.issue | 12 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INSERM | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | inserm-02983027 | |
| hal.version | 1 | |
| hal.export | false | |
| workflow.import.source | pubmed | |
| dc.rights.cc | CC BY-NC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cardiovascular%20Research&rft.date=2021-11-01&rft.volume=117&rft.issue=12&rft.spage=2489-2501&rft.epage=2489-2501&rft.eissn=1755-3245&rft.issn=1755-3245&rft.au=HOLLIER,%20Pierre-Louis&CHAPOULY,%20Candice&DIOP,%20Aissata&GUIMBAL,%20Sarah&CORNUAULT,%20Lauriane&rft.genre=article |
