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A new kit to detect Campylobacter species in stool specimens: the Orion GenRead Campylobacter(R)

dc.rights.licenseopenen_US
dc.contributor.authorBUISSONNIERE, A.
dc.contributor.authorBENEJAT, L.
dc.contributor.authorCHARRON, P.
dc.contributor.authorBESSEDE, E.
dc.contributor.authorLEHOURS, P.
hal.structure.identifierBordeaux population health [BPH]
dc.contributor.authorVALDENAIRE, Guillaume
dc.contributor.authorRICHER, O.
dc.contributor.authorMEGRAUD, F.
dc.date.accessioned2020-10-27T08:13:17Z
dc.date.available2020-10-27T08:13:17Z
dc.date.issued2018-08
dc.identifier.issn1435-4373 (Electronic) 0934-9723 (Linking)en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/11487
dc.description.abstractEnCampylobacter enteritis is the most frequent bacterial enteritis including in children. Its diagnosis suffers from the lack of sensitivity and delayed result of culture. Our aim was to test a new PCR-derived method for Campylobacter diagnosis in comparison to a composite reference. Patients presenting to the emergency ward of our hospital with enteric symptoms during the 2016 summer season were included. In addition to culture, an ELISA and an in-house real-time PCR were performed, as well as the new method (Orion GenRead Campylobacter) on all stool specimens. The composite reference used to consider a case positive for Campylobacter was either culture positive and in case of negative culture both the ELISA and real-time PCR positive. One hundred fifty patients were included, 64 being infants or children. There were 29 cases positive by the composite reference, with 19 of the 64 children (29.7%) and 10 of the 86 adults (11.6%). If performed alone, culture would have missed six cases. The Orion GenRead Campylobacter detected all the positives by the composite reference but also 12 cases negative by the composite reference (sensitivity 100%, specificity 90.1%). Given the characteristics of the new method, it can be used as a screening method for Campylobacter detection.
dc.language.isoENen_US
dc.subject.enIETO
dc.title.enA new kit to detect Campylobacter species in stool specimens: the Orion GenRead Campylobacter(R)
dc.title.alternativeEur J Clin Microbiol Infect Disen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1007/s10096-018-3288-5en_US
dc.subject.halSciences du Vivant [q-bio]/Santé publique et épidémiologieen_US
dc.identifier.pubmed29862421en_US
bordeaux.journalEuropean Journal of Clinical Microbiology and Infectious Diseasesen_US
bordeaux.page1585-1587en_US
bordeaux.volume37en_US
bordeaux.hal.laboratoriesBordeaux Population Health Research Center (BPH) - UMR 1219en_US
bordeaux.issue8en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03194238
hal.version1
hal.date.transferred2021-04-09T12:01:14Z
hal.exporttrue
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