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Enzymatically Shifting Nitroxides for EPR Spectroscopy and Overhauser-Enhanced Magnetic Resonance Imaging

hal.structure.identifierInstitut de Chimie Radicalaire [ICR]
dc.contributor.authorAUDRAN, Gérard
hal.structure.identifierInstitut de Chimie Radicalaire [ICR]
dc.contributor.authorBOSCO, Lionel
hal.structure.identifierInstitut de Chimie Radicalaire [ICR]
dc.contributor.authorBRÉMOND, Paul
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorFRANCONI, Jean-Michel
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorKOONJOO, Neha
hal.structure.identifierInstitut de Chimie Radicalaire [ICR]
dc.contributor.authorMARQUE, Sylvain R.A.
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorMASSOT, Philippe
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorMELLET, Philippe
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorPARZY, Elodie
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorTHIAUDIÈRE, Eric
dc.date.accessioned2021-10-07T16:30:29Z
dc.date.available2021-10-07T16:30:29Z
dc.date.issued2015-11-02
dc.identifier.issn1433-7851
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/112694
dc.description.abstractEnInvivo investigations of enzymatic processes using non-invasive approaches are a long-lasting challenge. Recently, we showed that Overhauser-enhanced MRI is suitable to such a purpose. A -phosphorylated nitroxide substrate prototype exhibiting keto-enol equilibrium upon enzymatic activity has been prepared. Upon enzymatic hydrolysis, a large variation of the phosphorus hyperfine coupling constant (a(P)=4G) was observed. The enzymatic activities of several enzymes were conveniently monitored by electronic paramagnetic resonance (EPR). Using a 0.2T MRI machine, invitro and invivo OMRI experiments were successfully performed, affording a 1200% enhanced MRI signal invitro, and a 600% enhanced signal invivo. These results highlight the enhanced imaging potential of these nitroxides upon specific enzymatic substrate-to-product conversion.
dc.language.isoen
dc.publisherWiley-VCH Verlag
dc.title.enEnzymatically Shifting Nitroxides for EPR Spectroscopy and Overhauser-Enhanced Magnetic Resonance Imaging
dc.typeArticle de revue
dc.identifier.doi10.1002/anie.201506267
dc.subject.halChimie
bordeaux.journalAngewandte Chemie International Edition
bordeaux.page13379 - 13384
bordeaux.volume54
bordeaux.hal.laboratoriesCentre de Résonance Magnétique des Systèmes Biologiques (CRMSB) - UMR 5536*
bordeaux.issue45
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-01413652
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01413652v1
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