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Online Quantification of Lactate Concentration in Microdialysate During Cerebral Activation Using 1H-MRS and Sensitive NMR Microcoil

hal.structure.identifierInstitut des Sciences Moléculaires [ISM]
dc.contributor.authorCRÉMILLIEUX, Yannick
hal.structure.identifierInstitut des Sciences Moléculaires [ISM]
dc.contributor.authorDUMONT, Ursule
hal.structure.identifierImagerie Moléculaire et Stratégies Théranostiques [IMoST]
dc.contributor.authorMAZUEL, Leslie
dc.contributor.authorSALVATI, Roberto
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorZHENDRE, Vanessa
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorRIZZITELLI, Silvia
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBLANC, Jordy
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorROUMES, Hélène
hal.structure.identifierInstitut des Sciences Moléculaires [ISM]
dc.contributor.authorPINAUD, Noël
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBOUZIER-SORE, Anne-Karine
dc.date.accessioned2021-10-07T16:29:58Z
dc.date.available2021-10-07T16:29:58Z
dc.date.issued2019-03-19
dc.identifier.issn1662-5102
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/112677
dc.description.abstractEnThe dynamic in vivo profiling of lactate is of uppermost importance in both neuroenergetics and neuroprotection fields, considering its central suspected role as a metabolic and signaling molecule. For this purpose, we implemented proton magnetic resonance spectroscopy (1 H-MRS) directly on brain microdialysate to monitor online the fluctuation of lactate contents during neuronal stimulation. Brain activation was obtained by right whisker stimulation of rats, which leads to the activation of the left barrel cortex area in which the microdialysis probe was implanted. The experimental protocol relies on the use of dedicated and sensitive home-made NMR microcoil able to perform lactate NMR profiling at submillimolar concentration. The MRS measurements of extracellular lactate concentration were performed inside a pre-clinical MRI scanner allowing simultaneous visualization of the correct location of the microprobe by MRI and detection of metabolites contained in the microdialysis by MRS. A 40% increase in lactate concentration was measured during whisker stimulation in the corresponding barrel cortex. This combination of microdialysis with online MRS/MRI provides a new approach to follow in vivo lactate fluctuations, and can be further implemented in physio-pathological conditions to get new insights on the role of lactate in brain metabolism and signaling.
dc.language.isoen
dc.publisherFrontiers
dc.subject.en1 H-MRS
dc.subject.enNMR microcoil
dc.subject.enmicrodialysis
dc.subject.enbarrel cortex
dc.subject.enlactate
dc.title.enOnline Quantification of Lactate Concentration in Microdialysate During Cerebral Activation Using 1H-MRS and Sensitive NMR Microcoil
dc.typeArticle de revue
dc.identifier.doi10.3389/fncel.2019.00089
dc.subject.halSciences du Vivant [q-bio]/Ingénierie biomédicale/Imagerie
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]
bordeaux.journalFrontiers in Cellular Neuroscience
bordeaux.volume13
bordeaux.hal.laboratoriesCentre de Résonance Magnétique des Systèmes Biologiques (CRMSB) - UMR 5536*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-02343562
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02343562v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers%20in%20Cellular%20Neuroscience&rft.date=2019-03-19&rft.volume=13&rft.eissn=1662-5102&rft.issn=1662-5102&rft.au=CR%C3%89MILLIEUX,%20Yannick&DUMONT,%20Ursule&MAZUEL,%20Leslie&SALVATI,%20Roberto&ZHENDRE,%20Vanessa&rft.genre=article


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