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An innovative flow cytometry method to screen human scFv-phages selected by in vivo phage-display in an animal model of atherosclerosis

dc.relation.isnodouble021347db-ea10-48ab-aed5-0e207ebd2157*
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorHEMADOU, Audrey
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorLAROCHE-TRAINEAU, Jeanny
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorANTOINE, Ségolène
hal.structure.identifierLFB Biotechnologies [Lille, France]
dc.contributor.authorMONDON, Philippe
hal.structure.identifierLFB Biotechnologies [Lille, France]
dc.contributor.authorFONTAYNE, Alexandre
dc.contributor.authorLE PRIOL, Yannick
hal.structure.identifierCentre Génomique Fonctionnelle Bordeaux [Bordeaux] [CGFB]
dc.contributor.authorCLAVEROL, Stephane
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorSANCHEZ, Stephane
hal.structure.identifierBaculovirus et Thérapie
dc.contributor.authorCERUTTI, Martine
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorOTTONES, Florence
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorCLOFENT-SANCHEZ, Gisèle
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorJACOBIN-VALAT, Marie-Josée
dc.date.accessioned2021-10-07T16:29:03Z
dc.date.available2021-10-07T16:29:03Z
dc.date.issued2018
dc.identifier.issn2045-2322
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/112628
dc.description.abstractEnAtherosclerosis is a chronic, progressive inflammatory disease that may develop into vulnerable lesions leading to thrombosis. This pathology is characterized by the deposition of lipids within the arterial wall and infiltration of immune cells leading to amplification of inflammation. Nowadays there is a rising interest to assess directly the molecular and cellular components that underlie the clinical condition of stroke and myocardial infarction. Single chain fragment variable (scFv)-phages issuing from a human combinatorial library were selected on the lesions induced in a rabbit model of atherosclerosis after three rounds of in vivo phage display. We further implemented a high-throughput flow cytometry method on rabbit protein extracts to individually test one thousand of scFv-phages. Two hundred and nine clones were retrieved on the basis of their specificity for atherosclerotic proteins. Immunohistochemistry assays confirmed the robustness of the designed cytometry protocol. Sequencing of candidates demonstrated their high diversity in VH and VL germline usage. The large number of candidates and their diversity open the way in the discovery of new biomarkers. Here, we successfully showed the capacity of combining in vivo phage display and high-throughput cytometry strategies to give new insights in in vivo targetable up-regulated biomarkers in atherosclerosis.
dc.language.isoen
dc.publisherNature Publishing Group
dc.title.enAn innovative flow cytometry method to screen human scFv-phages selected by in vivo phage-display in an animal model of atherosclerosis
dc.typeArticle de revue
dc.identifier.doi10.1038/s41598-018-33382-2
dc.subject.halSciences du Vivant [q-bio]/Ingénierie biomédicale
dc.subject.halSciences du Vivant [q-bio]/Immunologie
bordeaux.journalScientific Reports
bordeaux.page15016
bordeaux.volume8
bordeaux.hal.laboratoriesCentre de Résonance Magnétique des Systèmes Biologiques (CRMSB) - UMR 5536*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-02421128
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02421128v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2018&rft.volume=8&rft.spage=15016&rft.epage=15016&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=HEMADOU,%20Audrey&LAROCHE-TRAINEAU,%20Jeanny&ANTOINE,%20S%C3%A9gol%C3%A8ne&MONDON,%20Philippe&FONTAYNE,%20Alexandre&rft.genre=article


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