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Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part I: Optimisation of derivated histamine detection with coated-plates using optimal design

hal.structure.identifierInstitut des Sciences Moléculaires de Marseille [ISM2]
dc.contributor.authorCLAEYS-BRUNO, M.
hal.structure.identifierOrganisation moléculaire (évolution et matériaux fluores) [OMEMF]
dc.contributor.authorVANDENABEELE-TRAMBOUZE, O.
hal.structure.identifierInstitut des Sciences Moléculaires de Marseille [ISM2]
dc.contributor.authorSERGENT, M.
hal.structure.identifierLaboratoire de physique des interactions ondes matières [LPIOM]
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorGEFFARD, M.
hal.structure.identifierLaboratoire de physique des interactions ondes matières [LPIOM]
dc.contributor.authorBODET, D.
hal.structure.identifierLaboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux [L3AB]
hal.structure.identifierObservatoire aquitain des sciences de l'univers [OASU]
hal.structure.identifierLaboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
dc.contributor.authorDOBRIJEVIC, M.
hal.structure.identifierOrganisation moléculaire (évolution et matériaux fluores) [OMEMF]
dc.contributor.authorCOMMEYRAS, A.
hal.structure.identifierInstitut des Sciences Moléculaires de Marseille [ISM2]
dc.contributor.authorPHAN TAN LUU, R.
dc.date.issued2006
dc.identifier.issn0169-7439
dc.description.abstractEnUsing derivatization by chloroethylnitrosourea (CENU) a new strategy was used for raising antibodies directed against hapten (<300 Da) and the quantification of these haptens by ELISA. After raising antibodies directed against histamine, they were characterized and used for ELISA measurements. The development of a quantitative method needs an optimization of both detection and derivatization step. Experimental design methodology has been applied to optimize the conditions of the detection of the derivatized histamine. Methods and results were closely related.
dc.language.isoen
dc.publisherElsevier
dc.title.enMethodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part I: Optimisation of derivated histamine detection with coated-plates using optimal design
dc.typeArticle de revue
dc.identifier.doi10.1016/j.chemolab.2005.06.006
dc.subject.halPhysique [physics]/Astrophysique [astro-ph]/Cosmologie et astrophysique extra-galactique [astro-ph.CO]
dc.subject.halPlanète et Univers [physics]/Astrophysique [astro-ph]
bordeaux.journalChemometrics and Intelligent Laboratory Systems
bordeaux.page176-185
bordeaux.volume80
bordeaux.peerReviewedoui
hal.identifierhal-00023303
hal.version1
hal.popularnon
hal.audienceNon spécifiée
dc.subject.esExperimental design
dc.subject.esHybrid design
dc.subject.esAntibodies
dc.subject.esDerivatization
dc.subject.esHistamine analysis
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00023303v1
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