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Characterization of Isomers of Lipid A from Pseudomonas aeruginosa PAO1 by Liquid Chromatography with Tandem Mass Spectrometry with Higher-Energy Collisional Dissociation and Ultraviolet Photodissociation

dc.rights.licenseopenen_US
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBURE, Corinne
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLE SENECHAL, Caroline
dc.contributor.authorMACIAS, Luis
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorTOKARSKI, Caroline
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorVILAIN, Sebastien
dc.contributor.authorBRODBELT, Jennifer S.
dc.date.accessioned2021-07-01T09:53:09Z
dc.date.available2021-07-01T09:53:09Z
dc.date.issued2021
dc.identifier.issn0003-2700en_US
dc.identifier.otherhttps://pubs.acs.org/doi/10.1021/acs.analchem.0c05069?goto=supporting-infoen_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/94954
dc.description.abstractEnLipopolysaccharides (LPS) constitute the outermost layer of Gram-negative bacteria and consequently play an important role in bacterial infections. In order to address public health issues posed by Gram-negative bacteria, it is necessary to elucidate the structure of the molecular actors at the forefront of infections. LPS virulence and toxicity are partially modulated by lipid A, a hydrophobic saccharolipid that anchors LPS to the bacterial outer membrane. Understanding the lipid A structure is inherently intertwined with understanding its role as an endotoxin. Accordingly, several successful strategies incorporating tandem mass spectrometry have been applied toward the structural analysis of lipid A. Herein, a shotgun HCD strategy was applied toward the characterization of the lipid A profile of Pseudomonas aeruginosa PAO1. This analysis was enhanced by the development of an LC-MS/MS approach to eliminate isomeric signals in the MS/MS spectra that confounded characterization. Importantly, combining reverse phase chromatography with HCD and ultraviolet photodissociation analyses of the lipid A profile revealed the presence of previously unreported lipid A acyl chain positional isomers. Altogether, these strategies provide the most in-depth structural and molecular characterization of PAO1 lipid A to date.
dc.language.isoENen_US
dc.subject.enLipids
dc.subject.enMass spectrometry
dc.subject.enIons
dc.subject.enChemical structure
dc.subject.enAcyls
dc.title.enCharacterization of Isomers of Lipid A from Pseudomonas aeruginosa PAO1 by Liquid Chromatography with Tandem Mass Spectrometry with Higher-Energy Collisional Dissociation and Ultraviolet Photodissociation
dc.typeArticle de revueen_US
dc.identifier.doi10.1021/acs.analchem.0c05069en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalAnalytical Chemistryen_US
bordeaux.page4255-4262en_US
bordeaux.volume93en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248en_US
bordeaux.issue9en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03275570
hal.version1
hal.date.transferred2021-07-01T09:53:13Z
hal.exporttrue
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