Encapsulation of epsilon-Viniferin into Multi-Lamellar Liposomes: Development of a Rapid, Easy and Cost-Efficient Separation Method to Determine the Encapsulation Efficiency
| dc.rights.license | open | en_US |
| hal.structure.identifier | Unité de Recherche Oenologie [Villenave d'Ornon] [OENO] | |
| dc.contributor.author | BEAUMONT, Pauline | |
| hal.structure.identifier | Unité de Recherche Oenologie [Villenave d'Ornon] [OENO] | |
| dc.contributor.author | COURTOIS, Arnaud
IDREF: 092021301 | |
| hal.structure.identifier | Unité de Recherche Oenologie [Villenave d'Ornon] [OENO] | |
| dc.contributor.author | RICHARD, Tristan | |
| hal.structure.identifier | Unité de Recherche Oenologie [Villenave d'Ornon] [OENO] | |
| dc.contributor.author | KRISA, Stephanie | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | FAURE, Chrystel | |
| dc.date.accessioned | 2021-07-01T09:10:43Z | |
| dc.date.available | 2021-07-01T09:10:43Z | |
| dc.date.issued | 2021 | |
| dc.identifier.other | https://www.mdpi.com/1999-4923/13/4/566/s1 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/94951 | |
| dc.description.abstractEn | Onion-type multi-lamellar liposomes (MLLs), composed of a mixture of phosphatidylcholine and Tween 80, were analyzed for their ability to encapsulate epsilon-Viniferin (epsilon Vin), a resveratrol dimer. Their encapsulation efficiency (EE) was measured by UV-VIS spectroscopy using three different separation methods-ultracentrifugation, size exclusion chromatography, and a more original and advantageous one, based on adsorption filtration. The adsorption filtration method consists indeed of using syringe filters to retain the molecule of interest, and not the liposomes as usually performed. The process is rapid (less than 10 min), easy to handle, and inexpensive in terms of sample amount (around 2 mg of liposomes) and equipment (one syringe filter is required). Whatever the separation method, a similar EE value was determined, validating the proposed method. A total of 80% +/- 4% of epsilon Vin was found to be encapsulated leading to a 6.1% payload, roughly twice those reported for resveratrol-loaded liposomes. Finally, the release kinetics of epsilon Vin from MLLs was followed for a 77 day period, demonstrating a slow release of the polyphenol. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | encapsulation efficiency | |
| dc.subject.en | ε-Viniferin | |
| dc.subject.en | multi-lamellar liposomes | |
| dc.subject.en | adsorption filtration | |
| dc.subject.en | polyphenol | |
| dc.title.en | Encapsulation of epsilon-Viniferin into Multi-Lamellar Liposomes: Development of a Rapid, Easy and Cost-Efficient Separation Method to Determine the Encapsulation Efficiency | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.3390/pharmaceutics13040566 | en_US |
| dc.subject.hal | Chimie/Matériaux | en_US |
| bordeaux.journal | Pharmaceutics | en_US |
| bordeaux.page | 566 | en_US |
| bordeaux.volume | 13 | en_US |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | en_US |
| bordeaux.issue | 4 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | Bordeaux INP | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.institution | INRAE | |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.export | false | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Pharmaceutics&rft.date=2021&rft.volume=13&rft.issue=4&rft.spage=566&rft.epage=566&rft.au=BEAUMONT,%20Pauline&COURTOIS,%20Arnaud&RICHARD,%20Tristan&KRISA,%20Stephanie&FAURE,%20Chrystel&rft.genre=article |
