ABIDI, Wiem; ZOUHIR, Samira; CALEECHURN, Meryem; ROCHE, Stephane; KRASTEVA, Petya Violinova

doi:10.1126/sciadv.abd8049

Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

ZOUHIR, Samira
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

CALEECHURN, Meryem

Idioma

Article de revue

Este ítem está publicado en

Science Advances. 2021, vol. 7, n° 5

Resumen en inglés

Many free-living and pathogenic enterobacteria secrete biofilm-promoting cellulose using a multicomponent, envelope-embedded Bcs secretion system under the control of intracellular second messenger c-di-GMP. The molecular understanding of system assembly and cellulose secretion has been largely limited to the crystallographic studies of a distantly homologous BcsAB synthase tandem and a low-resolution reconstruction of an assembled macrocomplex that encompasses most of the inner membrane and cytosolic subunits and features an atypical layered architecture. Here, we present cryo-EM structures of the assembled Bcs macrocomplex, as well as multiple crystallographic snapshots of regulatory Bcs subcomplexes. The structural and functional data uncover the mechanism of asymmetric secretion system assembly and periplasmic crown polymerization and reveal unexpected subunit stoichiometry, multisite c-di-GMP recognition, and ATP-dependent regulation.< Leer menos

URI

https://oskar-bordeaux.fr/handle/20.500.12278/94950

DOI

http://dx.doi.org/10.1126/sciadv.abd8049

Link to research data

http://advances.sciencemag.org/content/suppl/2021/01/25/7.5.eabd8049.DC1

Centros de investigación

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248

Ver/Abrir

Metadatos

Compartir este ítem

Licencia de uso del documento

Architecture and regulation of an enterobacterial cellulose secretion system

Idioma

Este ítem está publicado en

Resumen en inglés

URI

DOI

Link to research data

Centros de investigación