BUSCAIL, Etienne; CHAUVET, Alexandre; QUINCY, Pascaline; DEGRANDI, Olivier; BUSCAIL, Camille; LAMRISSI, Isabelle; MORANVILLIER, Isabelle; CAUMONT, Charline; VERDON, Severine; BRISSON, Alain; MARTY, Marion; CHICHE, Laurence; LAURENT, Christophe; VENDRELY, Veronique; MOREAU-GAUDRY, Francois; BEDEL, Aurelie; DABERNAT, Sandrine

doi:10.1016/j.tranon.2019.07.009

BUSCAIL, Etienne
Inserm U1035, Biotherapies des Maladies Genetiques et Cancers, Univ Bordeaux, CHU de Bordeaux, Pole de Biologie et Pathologie

CHAUVET, Alexandre
Inserm U1035, Biotherapies des Maladies Genetiques et Cancers, Univ Bordeaux, CHU de Bordeaux, Pole de Biologie et Pathologie

QUINCY, Pascaline
Inserm U1035, Biotherapies des Maladies Genetiques et Cancers, Univ Bordeaux, CHU de Bordeaux, Pole de Biologie et Pathologie

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Translational Oncology. 2019, vol. 12, n° 11, p. 1395-1403

Résumé en anglais

Tumor-released extracellular vesicles (EVs) contain tumor-specific cargo distinguishing them from healthy EVs, and making them eligible as circulating biomarkers. Glypican 1 (GPC1)-positive exosome relevance as liquid biopsy elements is still debated. We carried out a prospective study to quantify GPC1-positive exosomes in sera from pancreatic ductal adenocarcinoma (PDAC) patients undergoing up-front surgery, as compared to controls including patients without cancer history and patients displaying pancreatic preneoplasic lesions. Sera were enriched in EVs, and exosomes were pulled down with anti-CD63 coupled magnetic beads. GPC1-positive bead percentages determined by flow cytometry were significantly higher in PDAC than in the control group. Diagnosis accuracy reached 78% (sensitivity 64% and specificity 90%), when results from peripheral and portal blood were combined. In association with echo-guided-ultrasound-fine-needle-aspiration (EUS-FNA) negative predictive value was 80% as compared to 33% for EUS-FNA only. This approach is clinically relevant as a companion test to the already available diagnostic tools, since patients with GPC1-positive exosomes in peripheral blood showed decreased tumor free survival.< Réduire

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https://oskar-bordeaux.fr/handle/20.500.12278/7910

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http://dx.doi.org/10.1016/j.tranon.2019.07.009

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CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248

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CD63-GPC1-Positive Exosomes Coupled with CA19-9 Offer Good Diagnostic Potential for Resectable Pancreatic Ductal Adenocarcinoma

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