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Interspecies differences with in vitro and in vivo models of vascular tissue engineering

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorRÉMY, Murielle
hal.structure.identifierCHU Bordeaux
dc.contributor.authorDURAND, Marlène
hal.structure.identifierIngénierie Moléculaire et Physiopathologie Articulaire [IMoPA]
dc.contributor.authorMENU, Patrick
hal.structure.identifierLaboratoire de tectonique, mécanique de la lithosphère [LTML (UMR_7578)]
dc.contributor.authorVOEGEL, Jean-Claude
hal.structure.identifierBanque de tissus et de cellules
dc.contributor.authorPONSOT, Jean-Francois
hal.structure.identifierCHU Bordeaux
dc.contributor.authorBERARD, Xavier
hal.structure.identifierUniversité Montesquieu - Bordeaux 4
dc.contributor.authorHARMAND, Marie-Francoise
hal.structure.identifierBiomateriaux et Reparation Tissulaire
hal.structure.identifierCHU Bordeaux
dc.contributor.authorBORDENAVE, Laurence
dc.date.accessioned2021-06-10T07:05:44Z
dc.date.available2021-06-10T07:05:44Z
dc.date.issued2013-12
dc.identifier.issn0142-9612
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/79037
dc.description.abstractEnIn arterial replacement there is a clear clinical need for a functional ă substitute possessing appropriate haemocompatible properties to be ă implanted as small diameter artery. Endothelial cell seeding constitutes ă an appreciated method to improve blood compatibility on the condition ă that cells firmly adhere to the support. Along this way, an innovative ă technique based on multilayered polyelectrolyte films (PEM) as cell ă adhesive substrate was previously validated in vitro and in vivo in a ă small-animal model. In this study, we extended the work on a larger ă animal (sheep) to validate furthermore the paradigm of PEM ă functionalization for vascular substitutes. We tested in vitro: the ă efficiency of PEM to induce endothelial progenitor differentiation in ă sheep endothelial cells; the ability of PEM to sustain cell ă proliferation and allow resistance to shear stress; the fate of ă PEM-coated de-endothelialized human saphenous veins under flow ă conditions, a prerequisite step before in vivo experiments. Despite in ă vitro differences we were encouraged by testing in vivo PEM-coated ă prosthesis as carotid replacement in sheep, but without success. In ă order to explain the implantation failure, an in vitro ă haemocompatibility evaluation was performed that highlighted ă interspecies differences able to explain, at least in part, the graft ă failure obtained. (C) 2013 Elsevier Ltd. All rights reserved.
dc.language.isoen
dc.publisherElsevier
dc.title.enInterspecies differences with in vitro and in vivo models of vascular tissue engineering
dc.typeArticle de revue
dc.identifier.doi10.1016/j.biomaterials.2013.07.091
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologie
bordeaux.journalBiomaterials
bordeaux.page9842-9852
bordeaux.volume34
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue38
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierhal-01464675
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01464675v1
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