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Strontium-doped hydroxyapatite polysaccharide materials effect on ectopic bone formation.

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorEHRET, Camille
hal.structure.identifierLaboratoire de Recherche Vasculaire Translationnelle [LVTS (UMR_S_1148 / U1148)]
dc.contributor.authorAID-LAUNAIS, Rachida
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSAGARDOY, Thomas
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSIADOUS, Robin
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBAREILLE, Reine
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorREY, S.
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorPECHEV, Stanislav
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorETIENNE, Laetitia
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorKALISKY, Jérome
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDE MONES, Erwan
hal.structure.identifierLaboratoire de Recherche Vasculaire Translationnelle [LVTS (UMR_S_1148 / U1148)]
dc.contributor.authorLETOURNEUR, Didier
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorAMEDEE, Joelle
dc.date.accessioned2021-06-10T07:05:42Z
dc.date.available2021-06-10T07:05:42Z
dc.date.issued2017-09-14
dc.identifier.issn1932-6203
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/79035
dc.description.abstractEnPrevious studies performed using polysaccharide-based matrices supplemented with hydroxyapatite (HA) particles showed their ability to form in subcutaneous and intramuscular sites a mineralized and osteoid tissue. Our objectives are to optimize the HA content in the matrix and to test the combination of HA with strontium (Sr-HA) to increase the matrix bioactivity. First, non-doped Sr-HA powders were combined to the matrix at three different ratios and were implanted subcutaneously for 2 and 4 weeks. Interestingly, matrices showed radiolucent properties before implantation. Quantitative analysis of micro-CT data evidenced a significant increase of mineralized tissue formed ectopically with time of implantation and allowed us to select the best ratio of HA to polysaccharides of 30% (w/w). Then, two Sr-substitution of 8% and 50% were incorporated in the HA powders (8Sr-HA and 50Sr-HA). Both Sr-HA were chemically characterized and dispersed in matrices. In vitro studies performed with human mesenchymal stem cells (MSCs) demonstrated the absence of cytotoxicity of the Sr-doped matrices whatever the amount of incorporated Sr. They also supported osteoblastic differentiation and activated the expression of one late osteoblastic marker involved in the mineralization process i.e. osteopontin. In vivo, subcutaneous implantation of these Sr-doped matrices induced osteoid tissue and blood vessels formation.
dc.language.isoen
dc.publisherPublic Library of Science
dc.title.enStrontium-doped hydroxyapatite polysaccharide materials effect on ectopic bone formation.
dc.typeArticle de revue
dc.identifier.doi10.1371/journal.pone.0184663
dc.subject.halChimie/Matériaux
bordeaux.journalPLoS ONE
bordeaux.pagee0184663 (21 p.)
bordeaux.volume12
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue9
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierhal-01591101
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01591101v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS%20ONE&rft.date=2017-09-14&rft.volume=12&rft.issue=9&rft.spage=e0184663%20(21%20p.)&rft.epage=e0184663%20(21%20p.)&rft.eissn=1932-6203&rft.issn=1932-6203&rft.au=EHRET,%20Camille&AID-LAUNAIS,%20Rachida&SAGARDOY,%20Thomas&SIADOUS,%20Robin&BAREILLE,%20Reine&rft.genre=article


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