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hal.structure.identifierCentre de recherche du CHU de Québec-Université Laval [CRCHUQ]
hal.structure.identifierUniversité Laval [Québec] [ULaval]
dc.contributor.authorKAWECKI, F
hal.structure.identifierCHU de Québec Research Center
hal.structure.identifierUniversité Laval [Québec] [ULaval]
dc.contributor.authorCLAFSHENKEL, W
hal.structure.identifierCHU de Québec Research Center
hal.structure.identifierUniversité Laval [Québec] [ULaval]
dc.contributor.authorAUGER, F.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOURGET, Jean-Michel
hal.structure.identifierCHU de Québec Research Center
hal.structure.identifierUniversité Laval [Québec] [ULaval]
dc.contributor.authorFRADETTE, J.
hal.structure.identifierInstitut National de la Santé et de la Recherche Médicale [INSERM]
dc.contributor.authorDEVILLARD, Raphael
dc.date.accessioned2021-06-10T07:05:30Z
dc.date.available2021-06-10T07:05:30Z
dc.date.issued2018-04-30
dc.identifier.issn1758-5082
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/79023
dc.description.abstractEnA major challenge during the engineering of voluminous bone tissues is to maintain cell viability in the central regions of the construct. In vitro prevascularization of bone substitutes relying on endothelial cell bioprinting has the potential to resolve this issue and to replicate the native bone microvasculature. Laser-assisted bioprinting (LAB) commonly uses biological layers of hydrogel, called 'biopapers', to support patterns of printed cells and constitute the basic units of the construct. The self-assembly approach of tissue engineering allows the production of biomimetic cell-derived bone extracellular matrix including living cells. We hypothesized that self-assembled osseous sheets can serve as living biopapers to support the LAB of human endothelial cells and thus guide tubule-like structure formation. Human umbilical vein endothelial cells were bioprinted on the surface of the biopapers following a predefined pattern of lines. The osseous biopapers showed relevant matrix mineralization and pro-angiogenic hallmarks. Our results revealed that formation of tubule-like structures was favored when the cellular orientation within the biopaper was parallel to the printed lines. Altogether, we validated that human osseous cell sheets can be used as biopapers for LAB, allowing the production of human prevascularized cell-based osseous constructs that can be relevant for autologous bone repair applications.
dc.language.isoen
dc.publisherIOP Publishing
dc.title.enSelf-assembled human osseous cell sheets as living biopapers for the laser-assisted bioprinting of human endothelial cells
dc.typeArticle de revue
dc.identifier.doi10.1088/1758-5090/aabd5b
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
bordeaux.journalBiofabrication
bordeaux.page035006
bordeaux.volume10
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue3
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierpasteur-01855956
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//pasteur-01855956v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biofabrication&rft.date=2018-04-30&rft.volume=10&rft.issue=3&rft.spage=035006&rft.epage=035006&rft.eissn=1758-5082&rft.issn=1758-5082&rft.au=KAWECKI,%20F&CLAFSHENKEL,%20W&AUGER,%20F.&BOURGET,%20Jean-Michel&FRADETTE,%20J.&rft.genre=article


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