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Self-crosslinked fibrous collagen/chitosan blends: Processing, properties evaluation and monitoring of degradation by bi-fluorescence imaging

hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorOLIVEIRA, P.N.
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorMONTEMBAULT, A.
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorSUDRE, G.
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorALCOUFFE, P.
hal.structure.identifieriLM - Formation, élaboration de nanomatériaux et cristaux [iLM - FENNEC]
dc.contributor.authorMARCON, Lionel
hal.structure.identifieriLM - Formation, élaboration de nanomatériaux et cristaux [iLM - FENNEC]
dc.contributor.authorGEHAN, Hélène
hal.structure.identifieriLM - Formation, élaboration de nanomatériaux et cristaux [iLM - FENNEC]
dc.contributor.authorLUX, François
hal.structure.identifierBiom'up
dc.contributor.authorALBESPY, K.
hal.structure.identifierBiom'up
dc.contributor.authorCENTIS, V.
hal.structure.identifierBiom'up
dc.contributor.authorCAMPOS, D.
hal.structure.identifierCIC Bordeaux
dc.contributor.authorROQUES, S.
hal.structure.identifierCIC Bordeaux
dc.contributor.authorMEULLE, M.
hal.structure.identifierCIC Bordeaux
dc.contributor.authorRENARD, M.
hal.structure.identifierCIC Bordeaux
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDURAND, M.
hal.structure.identifierCIC Bordeaux
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDENOST, Q.
hal.structure.identifierCIC Bordeaux
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBORDENAVE, L.
hal.structure.identifierVOXCAN
dc.contributor.authorVANDAMME, M.
hal.structure.identifierVOXCAN
dc.contributor.authorCHEREUL, E.
hal.structure.identifierMDB Texinov [MDB Texinov]
dc.contributor.authorVANDESTEENE, M.
hal.structure.identifierMDB Texinov [MDB Texinov]
dc.contributor.authorBOUCARD, N.
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorDAVID, L.
dc.date.accessioned2021-06-10T07:05:08Z
dc.date.available2021-06-10T07:05:08Z
dc.date.issued2019-06
dc.identifier.issn0141-8130
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/79007
dc.description.abstractEnPorous collagen/chitosan scaffolds with different Collagen:Chitosan (Coll:Ch) ratios were prepared by freeze-drying followed by self-crosslinking via dehydrothermal treatment (DHT) and characterized as biomaterials for tissue engineering. Cy7 and Cy5.5 fluorochromes were covalently grafted to collagen and chitosan, respectively. Thus, it was possible, using optical fluorescence imaging of the two fluorochromes, to simultaneously track their in vivo biodegradation, in a blend scaffold form. The fluorescence signal evolution, due to the bioresorption, corroborated with histological analysis. In vitro cytocompatibility of Coll:Ch blend scaffolds were evaluated with standardized tests. In addition, the scaffolds showed a highly interconnected porous structure. Extent of crosslinking was analyzed by convergent analysis using thermogravimetry, Fourier Transform Infrared Spectroscopy and PBS uptake. The variations observed with these techniques indicate strong interactions between collagen and chitosan (covalent and hydrogen bonds) promoted by the DHT. The mechanical properties were characterized to elucidate the impact of the different processing steps in the sample preparation (DHT, neutralization and sterilization by β-irradiation) and showed a robust processing scheme with low impact of Coll:Ch composition ratio.
dc.language.isoen
dc.publisherElsevier
dc.subject.enDehydrothermal treatment
dc.subject.enBi-fluorescence imaging
dc.subject.enCollagen:chitosan scaffolds
dc.title.enSelf-crosslinked fibrous collagen/chitosan blends: Processing, properties evaluation and monitoring of degradation by bi-fluorescence imaging
dc.typeArticle de revue
dc.identifier.doi10.1016/j.ijbiomac.2019.02.134
dc.subject.halChimie
dc.subject.halChimie/Polymères
bordeaux.journalInternational Journal of Biological Macromolecules
bordeaux.page353-367
bordeaux.volume131
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierhal-02335447
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02335447v1
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