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hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFENELON, Mathilde
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorETCHEBARNE, Marion
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSIADOUS, Robin
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorGREMARE, Agathe
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDURAND, Marlène
hal.structure.identifierService de gynécologie-obstétrique
dc.contributor.authorSENTILHES, Loïc
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorTORRES, Yoann
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorCATROS, Sylvain
hal.structure.identifierService de Chirurgie Orthopédique Traumatologique et Plastique [Besançon]
dc.contributor.authorGINDRAUX, Florelle
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorL'HEUREUX, Nicolas
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFRICAIN, Jean‐Christophe
dc.date.accessioned2021-06-10T07:04:16Z
dc.date.available2021-06-10T07:04:16Z
dc.date.issued2020-04-21
dc.identifier.issn1549-3296
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78963
dc.description.abstractEnThanks to its biological properties, the human amniotic membrane (HAM) can be used as a barrier membrane for guided bone regeneration (GBR). However, no study has assessed the influence of the preservation method of HAM for this application. This study aimed to establish the most suitable preservation method of HAM for GBR. Fresh (F), cryopreserved (C) lyophilized (L), and decellularized and lyophilized (DL) HAM were compared. The impact of preservation methods on collagen and glycosaminoglycans (GAG) content was evaluated using Masson's trichrome and alcian blue staining. Their suture retention strengths were assessed. In vitro, the osteogenic potential of human bone marrow mesenchymal stromal cells (hBMSCs) cultured on the four HAMs was evaluated using alkaline phosphatase staining and alizarin red quantification assay. In vivo, the effectiveness of fresh and preserved HAMs for GBR was assessed in a mice diaphyseal bone defect after 1 week or 1 month healing. Micro-CT and histomorphometric analysis were performed. The major structural components of HAM (collagen and GAG) were preserved whatever the preservation method used. The tearing strength of DL-HAM was significantly higher. In vitro, hBMSCs seeded on DL-HAM displayed a stronger ALP staining, and alizarin red staining quantification was significantly higher at Day 14. In vivo, L-HAM and DL-HAM significantly enhanced early bone regeneration. One month after the surgery, only DL-HAM slightly promoted bone regeneration. Several preserving methods of HAM have been studied for bone regeneration. Here, we have demonstrated that DL-HAM achieved the most promising results for GBR.
dc.language.isoen
dc.publisherWiley
dc.subject.enamniotic membrane
dc.subject.enbone
dc.subject.enin vivo
dc.subject.enprocessed amnion
dc.subject.entissue engineering.
dc.title.enAssessment of fresh and preserved amniotic membrane for guided bone regeneration in mice
dc.typeArticle de revue
dc.identifier.doi10.1002/jbm.a.36964
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalJournal of Biomedical Materials Research Part A
bordeaux.pageOnline ahead of print
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870401
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870401v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Biomedical%20Materials%20Research%20Part%20A&rft.date=2020-04-21&rft.spage=Online%20ahead%20of%20print&rft.epage=Online%20ahead%20of%20print&rft.eissn=1549-3296&rft.issn=1549-3296&rft.au=FENELON,%20Mathilde&ETCHEBARNE,%20Marion&SIADOUS,%20Robin&GREMARE,%20Agathe&DURAND,%20Marl%C3%A8ne&rft.genre=article


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