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Laser-assisted 3D bioprinting of exocrine pancreas spheroid models for cancer initiation study

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorHAKOBYAN, Davit
hal.structure.identifierPhysiopathologie du système nerveux central - Institut François Magendie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorMEDINA, Chantal
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDUSSERRE, Nathalie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSTACHOWICZ, Marie-Laure
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorHANDSCHIN, Charles
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFRICAIN, Jean-Christophe
hal.structure.identifierInstitut de médecine moléculaire de Rangueil [I2MR]
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorGUILLERMET-GUIBERT, Julie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorOLIVEIRA, Hugo
dc.date.accessioned2021-06-10T07:04:14Z
dc.date.available2021-06-10T07:04:14Z
dc.date.issued2020-07-01
dc.identifier.issn1758-5082
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78960
dc.description.abstractEnPancreatic ductal adenocarcinoma (PDAC) is the most common malignancy of the pancreas. It has shown a poor prognosis and a rising incidence in the developed world. Other pathologies associated with this tissue include pancreatitis, a risk condition for pancreatic cancer. The onset of both pancreatitis and pancreatic cancer follows a common pattern: exocrine pancreatic acinar cells undergo a transdifferentiation to duct cells that triggers a 3D restructuration of the pancreatic tissue. However, the exact mechanism underlying this process remains partially undefined. Further understanding the cellular events leading to PDAC could open new avenues in the development of novel therapeutic approaches. Since current 2D cell culture models fail to mimic the tridimensional complexity of the pancreatic tissue, new in vitro models are urgently needed. Here, we generated 3D pancreatic cell spheroid arrays using laser-assisted bioprinting and characterized their phenotypic evolution over time through image analysis and phenotypic characterization. We show that these bioprinted spheroids, composed of both acinar and ductal cells, can replicate the initial stages of PDAC development. This bioprinted miniaturized spheroid-based array model should prove useful for the study of the internal and external factors that contribute to the formation of precursor PDAC lesions and to cancer progression, and may therefore shed light on future PDAC therapy strategies.
dc.language.isoen
dc.publisherIOP Publishing
dc.title.enLaser-assisted 3D bioprinting of exocrine pancreas spheroid models for cancer initiation study
dc.typeArticle de revue
dc.identifier.doi10.1088/1758-5090/ab7cb8
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalBiofabrication
bordeaux.page035001
bordeaux.volume12
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue3
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870465
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870465v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biofabrication&rft.date=2020-07-01&rft.volume=12&rft.issue=3&rft.spage=035001&rft.epage=035001&rft.eissn=1758-5082&rft.issn=1758-5082&rft.au=HAKOBYAN,%20Davit&MEDINA,%20Chantal&DUSSERRE,%20Nathalie&STACHOWICZ,%20Marie-Laure&HANDSCHIN,%20Charles&rft.genre=article


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