Laser-assisted 3D bioprinting of exocrine pancreas spheroid models for cancer initiation study
MEDINA, Chantal
Physiopathologie du système nerveux central - Institut François Magendie
Bioingénierie tissulaire [BIOTIS]
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Physiopathologie du système nerveux central - Institut François Magendie
Bioingénierie tissulaire [BIOTIS]
MEDINA, Chantal
Physiopathologie du système nerveux central - Institut François Magendie
Bioingénierie tissulaire [BIOTIS]
Physiopathologie du système nerveux central - Institut François Magendie
Bioingénierie tissulaire [BIOTIS]
GUILLERMET-GUIBERT, Julie
Institut de médecine moléculaire de Rangueil [I2MR]
Bioingénierie tissulaire [BIOTIS]
< Leer menos
Institut de médecine moléculaire de Rangueil [I2MR]
Bioingénierie tissulaire [BIOTIS]
Idioma
en
Article de revue
Este ítem está publicado en
Biofabrication. 2020-07-01, vol. 12, n° 3, p. 035001
IOP Publishing
Resumen en inglés
Pancreatic ductal adenocarcinoma (PDAC) is the most common malignancy of the pancreas. It has shown a poor prognosis and a rising incidence in the developed world. Other pathologies associated with this tissue include ...Leer más >
Pancreatic ductal adenocarcinoma (PDAC) is the most common malignancy of the pancreas. It has shown a poor prognosis and a rising incidence in the developed world. Other pathologies associated with this tissue include pancreatitis, a risk condition for pancreatic cancer. The onset of both pancreatitis and pancreatic cancer follows a common pattern: exocrine pancreatic acinar cells undergo a transdifferentiation to duct cells that triggers a 3D restructuration of the pancreatic tissue. However, the exact mechanism underlying this process remains partially undefined. Further understanding the cellular events leading to PDAC could open new avenues in the development of novel therapeutic approaches. Since current 2D cell culture models fail to mimic the tridimensional complexity of the pancreatic tissue, new in vitro models are urgently needed. Here, we generated 3D pancreatic cell spheroid arrays using laser-assisted bioprinting and characterized their phenotypic evolution over time through image analysis and phenotypic characterization. We show that these bioprinted spheroids, composed of both acinar and ductal cells, can replicate the initial stages of PDAC development. This bioprinted miniaturized spheroid-based array model should prove useful for the study of the internal and external factors that contribute to the formation of precursor PDAC lesions and to cancer progression, and may therefore shed light on future PDAC therapy strategies.< Leer menos
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