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Layer‐by‐layer bioassembly of poly(lactic) acid membranes loaded with coculture of HBMSCs and EPCs improves vascularization in vivo

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorGUDURIC, Vera
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSIADOUS, Robin
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBABILOTTE, Joanna
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSEIMBILLE, Maxime
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBAREILLE, Reine
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorREY, Sylvie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorTHÉBAUD, Noelie
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorLE NIHOUANNEN, Damien
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFRICAIN, Jean‐Christophe
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDEVILLARD, Raphael
hal.structure.identifierFaculty of Sciences [Novi Sad]
dc.contributor.authorLUZANIN, Ognjan
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorCATROS, Sylvain
dc.date.accessioned2021-06-10T07:04:11Z
dc.date.available2021-06-10T07:04:11Z
dc.date.issued2019-08-23
dc.identifier.issn1549-3296
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78958
dc.description.abstractEnLayer-by-layer (LBL) BioAssembly method was developed to enhance the control of cell distribution within 3D scaffolds for tissue engineering applications. The objective of this study was to evaluate in vivo the development of blood vessels within LBL bioassembled membranes seeded with human primary cells, and to compare it to cellularized massive scaffolds. Poly(lactic) acid (PLA) membranes fabricated by fused deposition modeling were seeded with monocultures of human bone marrow stromal cells or with cocultures of these cells and endothelial progenitor cells. Then, four cellularized membranes were assembled in LBL constructs. Early osteoblastic and endothelial cell differentiation markers, alkaline phosphatase, and von Willebrand's factor, were expressed in all layers of assemblies in homogenous manner. The same kind of LBL assemblies as well as cellularized massive scaffolds was implanted subcutaneously in mice. Human cells were observed in all scaffolds seeded with cells, but not in the inner parts of massive scaffolds. There were significantly more blood vessels observed in LBL bioassemblies seeded with cocultures compared to all other samples. LBL bioassembly of PLA membranes seeded with a coculture of human cells is an efficient method to obtain homogenous cell distribution and blood vessel formation within the entire volume of a 3D composite scaffold.
dc.language.isoen
dc.publisherWiley
dc.subject.enbiofabrication
dc.subject.enin vivo
dc.subject.enlayer-by-layer
dc.subject.entissue engineering
dc.subject.envascularization
dc.title.enLayer‐by‐layer bioassembly of poly(lactic) acid membranes loaded with coculture of HBMSCs and EPCs improves vascularization in vivo
dc.typeArticle de revue
dc.identifier.doi10.1002/jbm.a.36769
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalJournal of Biomedical Materials Research Part A
bordeaux.page2629-2642
bordeaux.volume107
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue12
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870482
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870482v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Biomedical%20Materials%20Research%20Part%20A&rft.date=2019-08-23&rft.volume=107&rft.issue=12&rft.spage=2629-2642&rft.epage=2629-2642&rft.eissn=1549-3296&rft.issn=1549-3296&rft.au=GUDURIC,%20Vera&SIADOUS,%20Robin&BABILOTTE,%20Joanna&SEIMBILLE,%20Maxime&BAREILLE,%20Reine&rft.genre=article


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