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In situ prevascularization designed by laser-assisted bioprinting: effect on bone regeneration

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierPôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
dc.contributor.authorKÉROURÉDAN, Olivia
hal.structure.identifierART BioPrint
dc.contributor.authorHAKOBYAN, Davit
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorRÉMY, Murielle
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierPôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
dc.contributor.authorZIANE, Sophia
hal.structure.identifierART BioPrint
dc.contributor.authorDUSSERRE, Nathalie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierPôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
hal.structure.identifierART BioPrint
dc.contributor.authorFRICAIN, Jean-Christophe
hal.structure.identifierCIC Bordeaux
dc.contributor.authorDELMOND, Samantha
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorTHÉBAUD, Noëlie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierPôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
dc.contributor.authorDEVILLARD, Raphael
dc.date.accessioned2021-06-10T07:04:09Z
dc.date.available2021-06-10T07:04:09Z
dc.date.issued2019-07-03
dc.identifier.issn1758-5082
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78956
dc.description.abstractEnVascularization plays a crucial role in bone formation and regeneration process. Development of a functional vasculature to improve survival and integration of tissue-engineered bone substitutes remains a major challenge. Biofabrication technologies, such as bioprinting, have been introduced as promising alternatives to overcome issues related to lack of prevascularization and poor organization of vascular networks within the bone substitutes. In this context, this study aimed at organizing endothelial cells in situ, in a mouse calvaria bone defect, to generate a prevascularization with a defined architecture, and promote in vivo bone regeneration. Laser-assisted bioprinting (LAB) was used to pattern Red Fluorescent Protein-labeled endothelial cells into a mouse calvaria bone defect of critical size, filled with collagen containing mesenchymal stem cells and vascular endothelial growth factor. LAB technology allowed safe and controlled in vivo printing of different cell patterns. In situ printing of endothelial cells gave rise to organized microvascular networks into bone defects. At two months, vascularization rate (vr) and bone regeneration rate (br) showed statistically significant differences between the 'random seeding' condition and both 'disc' pattern (vr = +203.6%; br = +294.1%) and 'crossed circle' pattern (vr = +355%; br = +602.1%). These results indicate that in vivo LAB is a valuable tool to introduce in situ prevascularization with a defined configuration and promote bone regeneration.
dc.language.isoen
dc.publisherIOP Publishing
dc.title.enIn situ prevascularization designed by laser-assisted bioprinting: effect on bone regeneration
dc.typeArticle de revue
dc.identifier.doi10.1088/1758-5090/ab2620
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalBiofabrication
bordeaux.page045002
bordeaux.volume11
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue4
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870502
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870502v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biofabrication&rft.date=2019-07-03&rft.volume=11&rft.issue=4&rft.spage=045002&rft.epage=045002&rft.eissn=1758-5082&rft.issn=1758-5082&rft.au=K%C3%89ROUR%C3%89DAN,%20Olivia&HAKOBYAN,%20Davit&R%C3%89MY,%20Murielle&ZIANE,%20Sophia&DUSSERRE,%20Nathalie&rft.genre=article


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