Mostrar el registro sencillo del ítem

Towards an in vitro model of glomerular barrier unit with an innovative bioassembly method

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFLÉGEAU, Killian
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierService de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
dc.contributor.authorRUBIN, Sébastien
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierService de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
dc.contributor.authorMUCHA, Simon
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBUR, Pauline
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorPRÉTERRE, Julie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSIADOUS, Robin
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorL’AZOU, Béatrice
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierPôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
dc.contributor.authorFRICAIN, Jean-Christophe
hal.structure.identifierService de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
dc.contributor.authorCOMBE, Christian
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierPôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
dc.contributor.authorDEVILLARD, Raphael
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorKALISKY, Jérome
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierService de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
dc.contributor.authorRIGOTHIER, Claire
dc.date.accessioned2021-06-10T07:04:08Z
dc.date.available2021-06-10T07:04:08Z
dc.date.issued2020-02-01
dc.identifier.issn0931-0509
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78955
dc.description.abstractEnBackground: The development of an artificial glomerular unit may be pivotal for renal pathophysiology studies at a multicellular scale. Using a tissue engineering approach, we aimed to reproduce in part the specific glomerular barrier architecture by manufacturing a glomerular microfibre (Mf).Methods: Immortalized human glomerular cell lines of endothelial cells (GEnCs) and podocytes were used. Cells and a three-dimensional (3D) matrix were characterized by immunofluorescence with confocal analysis, Western blot and polymerase chain reaction. Optical and electron microscopy were used to study Mf and cell shapes. We also analysed cell viability and cell metabolism within the 3D construct at 14 days.Results: Using the Mf manufacturing method, we repeatedly obtained a cellularized Mf sorting human glomerular cells in 3D. Around a central structure made of collagen I, we obtained an internal layer composed of GEnC, a newly formed glomerular basement membrane rich in α5 collagen IV and an external layer of podocytes. The cell concentration, optimal seeding time and role of physical stresses were modulated to obtain the Mf. Cell viability and expression of specific proteins (nephrin, synaptopodin, vascular endothelial growth factor receptor 2 (VEGFR2) and von Willebrandt factor (vWF)) were maintained for 19 days in the Mf system. Mf ultrastructure, observed with EM, had similarities with the human glomerular barrier.Conclusion: In summary, with our 3D bio-engineered glomerular fibre, GEnC and podocytes produced a glomerular basement membrane. In the future, this glomerular Mf will allow us to stud
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subject.enbioassembly
dc.subject.englomerular barrier
dc.subject.englomerular endothelial cells
dc.subject.enpodocytes
dc.subject.entissue engineering
dc.title.enTowards an in vitro model of glomerular barrier unit with an innovative bioassembly method
dc.typeArticle de revue
dc.identifier.doi10.1093/ndt/gfz094
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalNephrology Dialysis Transplantation
bordeaux.page240-250
bordeaux.volume35
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue2
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870507
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870507v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nephrology%20Dialysis%20Transplantation&rft.date=2020-02-01&rft.volume=35&rft.issue=2&rft.spage=240-250&rft.epage=240-250&rft.eissn=0931-0509&rft.issn=0931-0509&rft.au=FL%C3%89GEAU,%20Killian&RUBIN,%20S%C3%A9bastien&MUCHA,%20Simon&BUR,%20Pauline&PR%C3%89TERRE,%20Julie&rft.genre=article


Archivos en el ítem

ArchivosTamañoFormatoVer

No hay archivos asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem