Towards an in vitro model of glomerular barrier unit with an innovative bioassembly method
RUBIN, Sébastien
Bioingénierie tissulaire [BIOTIS]
Service de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
Bioingénierie tissulaire [BIOTIS]
Service de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
MUCHA, Simon
Bioingénierie tissulaire [BIOTIS]
Service de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
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Bioingénierie tissulaire [BIOTIS]
Service de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
RUBIN, Sébastien
Bioingénierie tissulaire [BIOTIS]
Service de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
Bioingénierie tissulaire [BIOTIS]
Service de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
MUCHA, Simon
Bioingénierie tissulaire [BIOTIS]
Service de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
Bioingénierie tissulaire [BIOTIS]
Service de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
FRICAIN, Jean-Christophe
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
DEVILLARD, Raphael
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
Bioingénierie tissulaire [BIOTIS]
Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux]
RIGOTHIER, Claire
Bioingénierie tissulaire [BIOTIS]
Service de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
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Bioingénierie tissulaire [BIOTIS]
Service de Néphrologie Transplantation, Dialyse et Aphérèse [CHU Bordeaux]
Idioma
en
Article de revue
Este ítem está publicado en
Nephrology Dialysis Transplantation. 2020-02-01, vol. 35, n° 2, p. 240-250
Oxford University Press (OUP)
Resumen en inglés
Background: The development of an artificial glomerular unit may be pivotal for renal pathophysiology studies at a multicellular scale. Using a tissue engineering approach, we aimed to reproduce in part the specific ...Leer más >
Background: The development of an artificial glomerular unit may be pivotal for renal pathophysiology studies at a multicellular scale. Using a tissue engineering approach, we aimed to reproduce in part the specific glomerular barrier architecture by manufacturing a glomerular microfibre (Mf).Methods: Immortalized human glomerular cell lines of endothelial cells (GEnCs) and podocytes were used. Cells and a three-dimensional (3D) matrix were characterized by immunofluorescence with confocal analysis, Western blot and polymerase chain reaction. Optical and electron microscopy were used to study Mf and cell shapes. We also analysed cell viability and cell metabolism within the 3D construct at 14 days.Results: Using the Mf manufacturing method, we repeatedly obtained a cellularized Mf sorting human glomerular cells in 3D. Around a central structure made of collagen I, we obtained an internal layer composed of GEnC, a newly formed glomerular basement membrane rich in α5 collagen IV and an external layer of podocytes. The cell concentration, optimal seeding time and role of physical stresses were modulated to obtain the Mf. Cell viability and expression of specific proteins (nephrin, synaptopodin, vascular endothelial growth factor receptor 2 (VEGFR2) and von Willebrandt factor (vWF)) were maintained for 19 days in the Mf system. Mf ultrastructure, observed with EM, had similarities with the human glomerular barrier.Conclusion: In summary, with our 3D bio-engineered glomerular fibre, GEnC and podocytes produced a glomerular basement membrane. In the future, this glomerular Mf will allow us to stud< Leer menos
Palabras clave en inglés
bioassembly
glomerular barrier
glomerular endothelial cells
podocytes
tissue engineering
Orígen
Importado de HalCentros de investigación