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In-vitro and in-vivo design and validation of an injectable polysaccharide-hydroxyapatite composite material for sinus floor augmentation

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFRICAIN, Jean-Christophe
ORCID: 0000-0001-7855-6437
hal.structure.identifierLaboratoire de Recherche Vasculaire Translationnelle [LVTS (UMR_S_1148 / U1148)]
dc.contributor.authorAID, R.
hal.structure.identifierLaboratoire de Recherche Vasculaire Translationnelle [LVTS (UMR_S_1148 / U1148)]
dc.contributor.authorLANOUAR, S.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorMAUREL, D.B.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorLE NIHOUANNEN, Damien
ORCID: 0000-0003-1539-5183
IDREF: 102521751
hal.structure.identifierCIC Bordeaux
dc.contributor.authorDELMOND, S.
hal.structure.identifierLaboratoire de Recherche Vasculaire Translationnelle [LVTS (UMR_S_1148 / U1148)]
dc.contributor.authorLETOURNEUR, D.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorAMEDEE, Joelle
ORCID: 0000-0002-4888-0129
IDREF: 87485605
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorCATROS, Sylvain
ORCID: 0000-0002-3419-3467
dc.date.accessioned2021-06-10T07:04:05Z
dc.date.available2021-06-10T07:04:05Z
dc.date.issued2018-07
dc.identifier.issn0109-5641
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78952
dc.description.abstractEnObjective: Polysaccharide-based composite matrices consisting of natural polysaccharides, pullulan and dextran supplemented with hydroxyapatite (Matrix-HA) have recently been developed. The principal objective of this study was to evaluate the capacities of this composite material to promote new bone formation in a sinus lift model in the sheep. Secondary objectives were to evaluate in vitro properties of the material regarding cell adhesion and proliferation.Methods: In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.Results: In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture. In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation. CBCT analyses at the implantation time revealed the radiolucent properties of these matrices. Quantitative analysis showed an increase of a dense mineralized tissue in the Matrix-HA group up to 3 months of implantation. The mineralized volume over total volume after 6 months reached comparable values to those obtained for Bio-Oss® used as positive control. Histological examination confirmed that the Matrix-HA did not induce any long term inflammatory events, and promoted direct contact between the osteoid tissue and lamellar bone structures and beads. After 6 months, we observed a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials.Significance: In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.
dc.language.isoen
dc.publisherElsevier
dc.subject.enBone regeneration
dc.subject.enInjectable bone substitute
dc.subject.enPolysaccharide composites
dc.subject.enSheep model
dc.subject.enSinus floor augmentation.
dc.title.enIn-vitro and in-vivo design and validation of an injectable polysaccharide-hydroxyapatite composite material for sinus floor augmentation
dc.typeArticle de revue
dc.identifier.doi10.1016/j.dental.2018.03.021
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalDental Materials
bordeaux.page1024-1035
bordeaux.volume34
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue7
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870583
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870583v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Dental%20Materials&rft.date=2018-07&rft.volume=34&rft.issue=7&rft.spage=1024-1035&rft.epage=1024-1035&rft.eissn=0109-5641&rft.issn=0109-5641&rft.au=FRICAIN,%20Jean-Christophe&AID,%20R.&LANOUAR,%20S.&MAUREL,%20D.B.&LE%20NIHOUANNEN,%20Damien&rft.genre=article


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