The proangiogenic potential of a novel calcium releasing composite biomaterial: Orthotopic in vivo evaluation
CASTANO, Oscar
Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona [UAB]
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Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona [UAB]
CASTANO, Oscar
Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona [UAB]
Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona [UAB]
CLIFT, Douglas
Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
MARTI-MUNOZ, Joan
Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
BATISTA, Marc
Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
PLANELL, Josep
Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona [UAB]
Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona [UAB]
ENGEL, Elisabeth
Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
Universitat Politècnica de Catalunya = Université polytechnique de Catalogne [Barcelona] [UPC]
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Institute for Bioengineering of Catalonia [Barcelona] [IBEC]
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine [CIBER-BBN]
Universitat Politècnica de Catalunya = Université polytechnique de Catalogne [Barcelona] [UPC]
Language
en
Article de revue
This item was published in
Acta Biomaterialia. 2017-05, vol. 54, p. 377-385
Elsevier
English Abstract
Insufficient angiogenesis remains a major hurdle in current bone tissue engineering strategies. An extensive body of work has focused on the use of angiogenic factors or endothelial progenitor cells. However, these approaches ...Read more >
Insufficient angiogenesis remains a major hurdle in current bone tissue engineering strategies. An extensive body of work has focused on the use of angiogenic factors or endothelial progenitor cells. However, these approaches are inherently complex, in terms of regulatory and methodologic implementation, and present a high cost. We have recently demonstrate the potential of electrospun poly(lactic acid) (PLA) fiber-based membranes, containing calcium phosphate (CaP) ormoglass particles, to elicit angiogenesis in vivo, in a subcutaneous model in mice. Here we have devised an injectable composite, containing CaP glass-ceramic particles, dispersed within a (Hydroxypropyl)methyl cellulose (HPMC) matrix, with the capacity to release calcium in a more sustained fashion. We show that by tuning the release of calcium in vivo, in a rat bone defect model, we could improve both bone formation and increase angiogenesis. The bone regeneration kinetics was dependent on the Ca2+ release rate, with the faster Ca2+ release composite gel showing improved bone repair at 3weeks, in relation to control. In the same line, improved angiogenesis could be observed for the same gel formulation at 6weeks post implantation. This methodology allows to integrate two fundamental processes for bone tissue regeneration while using a simple, cost effective, and safe approach.Statement of significance: In current bone tissue engineering approaches the achievement of sufficient angiogenesis, during tissue regeneration, is a major limitation in order to attain full tissue functionality. Recently, we have shown that calcium ions, released by the degradation of calcium phosphate ormoglasses (CaP), are effective angiogenic promoters, in both in vitro and in a subcutaneous implantation model. Here, we devised an injectable composite, containing CaP glass-ceramic particles, dispersed within a HPMC matrix, enabling the release of calcium in a more sustained fashion. We show that by tuning the release of calcium in vivo, in a rat bone defect model, we could improve both bone formation and increase angiogenesis. This simple and cost effective approach holds great promise to translate to the clinics.Read less <
English Keywords
Angiogenesis
Bone regeneration
Calcium phosphate ormoglasses
Origin
Hal importedCollections