Afficher la notice abrégée

Micropatterning of endothelial cells to create a capillary-like network with defined architecture by laser-assisted bioprinting

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierPôle d’Odontologie et de Santé Buccale
dc.contributor.authorKÉROURÉDAN, Olivia
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierEnergie, matériaux et télécommunication
dc.contributor.authorBOURGET, Jean-Michel
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorREMY, Murielle
hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorCRAUSTE-MANCIET, Sylvie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorKALISKY, Jérome
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierPôle d’Odontologie et de Santé Buccale
dc.contributor.authorCATROS, Sylvain
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierPôle d’Odontologie et de Santé Buccale
dc.contributor.authorTHÉBAUD, Noëlie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierPôle d’Odontologie et de Santé Buccale
dc.contributor.authorDEVILLARD, Raphael
dc.date.accessioned2021-06-10T07:03:52Z
dc.date.available2021-06-10T07:03:52Z
dc.date.issued2019-02
dc.identifier.issn0957-4530
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78939
dc.description.abstractEnDevelopment of a microvasculature into tissue-engineered bone substitutes represents a current challenge. Seeding of endothelial cells in an appropriate environment can give rise to a capillary-like network to enhance prevascularization of bone substitutes. Advances in biofabrication techniques, such as bioprinting, could allow to precisely define a pattern of endothelial cells onto a biomaterial suitable for in vivo applications. The aim of this study was to produce a microvascular network following a defined pattern and preserve it while preparing the surface to print another layer of endothelial cells. We first optimise the bioink cell concentration and laser printing parameters and then develop a method to allow endothelial cells to survive between two collagen layers. Laser-assisted bioprinting (LAB) was used to pattern lines of tdTomato-labeled endothelial cells cocultured with mesenchymal stem cells seeded onto a collagen hydrogel. Formation of capillary-like structures was dependent on a sufficient local density of endothelial cells. Overlay of the pattern with collagen I hydrogel containing vascular endothelial growth factor (VEGF) allowed capillary-like structures formation and preservation of the printed pattern over time. Results indicate that laser-assisted bioprinting is a valuable technique to pre-organize endothelial cells into high cell density pattern in order to create a vascular network with defined architecture in tissue-engineered constructs based on collagen hydrogel.
dc.language.isoen
dc.publisherSpringer Verlag
dc.title.enMicropatterning of endothelial cells to create a capillary-like network with defined architecture by laser-assisted bioprinting
dc.typeArticle de revue
dc.identifier.doi10.1007/s10856-019-6230-1
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalJournal of Materials Science: Materials in Medicine
bordeaux.page28
bordeaux.volume30
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue2
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870947
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870947v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Materials%20Science:%20Materials%20in%20Medicine&rft.date=2019-02&rft.volume=30&rft.issue=2&rft.spage=28&rft.epage=28&rft.eissn=0957-4530&rft.issn=0957-4530&rft.au=K%C3%89ROUR%C3%89DAN,%20Olivia&BOURGET,%20Jean-Michel&REMY,%20Murielle&CRAUSTE-MANCIET,%20Sylvie&KALISKY,%20J%C3%A9rome&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée